A birefringence, electron microscopy, and histochemical survey of chromosomal structure in the sperm nuclei of an echinothurid sea urchin, Araeosoma owstoni

Shonan Amemiya, Isao Uemura, Tadashi Shinkai, Hidemi Sato

Research output: Contribution to journalArticle

Abstract

The mature sperm head of Araeosoma owstoni, an echinothurid sea urchin, showed positive birefringence reflecting that the overall orientation of DNA molecules was semiperpendicular toward the nuclear axis of the sperm head. Transmission electron microscopical observation of sperm in this species revealed a highly electron-dense cylindrical coil with an empty central core extending along the major axis of the sperm head. This coil had seven to eight turns along its entire length of 3.5 μm. The maximum width was 0.35 μm near the distal end of the nucleus, and the minimum width was 0.17 μm near the apical end. Lamellar substructures were also present in the sperm nucleus, appearing at the periphery of the electron-dense cylinder in a radial manner. Staining with Feulgen's reaction and acid-orcein indicated that the coil was probably composed of sperm chromosomes.

Original languageEnglish
Pages (from-to)446-453
Number of pages8
JournalExperimental Cell Research
Volume179
Issue number2
DOIs
Publication statusPublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

Cite this

A birefringence, electron microscopy, and histochemical survey of chromosomal structure in the sperm nuclei of an echinothurid sea urchin, Araeosoma owstoni. / Amemiya, Shonan; Uemura, Isao; Shinkai, Tadashi; Sato, Hidemi.

In: Experimental Cell Research, Vol. 179, No. 2, 1988, p. 446-453.

Research output: Contribution to journalArticle

@article{b5d7dff9e8dd4624aa07d71f0940fe3d,
title = "A birefringence, electron microscopy, and histochemical survey of chromosomal structure in the sperm nuclei of an echinothurid sea urchin, Araeosoma owstoni",
abstract = "The mature sperm head of Araeosoma owstoni, an echinothurid sea urchin, showed positive birefringence reflecting that the overall orientation of DNA molecules was semiperpendicular toward the nuclear axis of the sperm head. Transmission electron microscopical observation of sperm in this species revealed a highly electron-dense cylindrical coil with an empty central core extending along the major axis of the sperm head. This coil had seven to eight turns along its entire length of 3.5 μm. The maximum width was 0.35 μm near the distal end of the nucleus, and the minimum width was 0.17 μm near the apical end. Lamellar substructures were also present in the sperm nucleus, appearing at the periphery of the electron-dense cylinder in a radial manner. Staining with Feulgen's reaction and acid-orcein indicated that the coil was probably composed of sperm chromosomes.",
author = "Shonan Amemiya and Isao Uemura and Tadashi Shinkai and Hidemi Sato",
year = "1988",
doi = "10.1016/0014-4827(88)90282-0",
language = "English",
volume = "179",
pages = "446--453",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - A birefringence, electron microscopy, and histochemical survey of chromosomal structure in the sperm nuclei of an echinothurid sea urchin, Araeosoma owstoni

AU - Amemiya, Shonan

AU - Uemura, Isao

AU - Shinkai, Tadashi

AU - Sato, Hidemi

PY - 1988

Y1 - 1988

N2 - The mature sperm head of Araeosoma owstoni, an echinothurid sea urchin, showed positive birefringence reflecting that the overall orientation of DNA molecules was semiperpendicular toward the nuclear axis of the sperm head. Transmission electron microscopical observation of sperm in this species revealed a highly electron-dense cylindrical coil with an empty central core extending along the major axis of the sperm head. This coil had seven to eight turns along its entire length of 3.5 μm. The maximum width was 0.35 μm near the distal end of the nucleus, and the minimum width was 0.17 μm near the apical end. Lamellar substructures were also present in the sperm nucleus, appearing at the periphery of the electron-dense cylinder in a radial manner. Staining with Feulgen's reaction and acid-orcein indicated that the coil was probably composed of sperm chromosomes.

AB - The mature sperm head of Araeosoma owstoni, an echinothurid sea urchin, showed positive birefringence reflecting that the overall orientation of DNA molecules was semiperpendicular toward the nuclear axis of the sperm head. Transmission electron microscopical observation of sperm in this species revealed a highly electron-dense cylindrical coil with an empty central core extending along the major axis of the sperm head. This coil had seven to eight turns along its entire length of 3.5 μm. The maximum width was 0.35 μm near the distal end of the nucleus, and the minimum width was 0.17 μm near the apical end. Lamellar substructures were also present in the sperm nucleus, appearing at the periphery of the electron-dense cylinder in a radial manner. Staining with Feulgen's reaction and acid-orcein indicated that the coil was probably composed of sperm chromosomes.

UR - http://www.scopus.com/inward/record.url?scp=0024261703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024261703&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(88)90282-0

DO - 10.1016/0014-4827(88)90282-0

M3 - Article

C2 - 3191949

AN - SCOPUS:0024261703

VL - 179

SP - 446

EP - 453

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -