TY - JOUR
T1 - A birefringence, electron microscopy, and histochemical survey of chromosomal structure in the sperm nuclei of an echinothurid sea urchin, Araeosoma owstoni
AU - Amemiya, Shonan
AU - Uemura, Isao
AU - Shinkai, Tadashi
AU - Sato, Hidemi
N1 - Funding Information:
We express our sincere thanks to Dr. Y. Hamaguchi of Tokyo Institute of Technology for permittingu s to use his polarizing microscope.T his work was supportedb y a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan and a Fund for Special Research Project from Tokyo Metropolitan Government.
PY - 1988/12
Y1 - 1988/12
N2 - The mature sperm head of Araeosoma owstoni, an echinothurid sea urchin, showed positive birefringence reflecting that the overall orientation of DNA molecules was semiperpendicular toward the nuclear axis of the sperm head. Transmission electron microscopical observation of sperm in this species revealed a highly electron-dense cylindrical coil with an empty central core extending along the major axis of the sperm head. This coil had seven to eight turns along its entire length of 3.5 μm. The maximum width was 0.35 μm near the distal end of the nucleus, and the minimum width was 0.17 μm near the apical end. Lamellar substructures were also present in the sperm nucleus, appearing at the periphery of the electron-dense cylinder in a radial manner. Staining with Feulgen's reaction and acid-orcein indicated that the coil was probably composed of sperm chromosomes.
AB - The mature sperm head of Araeosoma owstoni, an echinothurid sea urchin, showed positive birefringence reflecting that the overall orientation of DNA molecules was semiperpendicular toward the nuclear axis of the sperm head. Transmission electron microscopical observation of sperm in this species revealed a highly electron-dense cylindrical coil with an empty central core extending along the major axis of the sperm head. This coil had seven to eight turns along its entire length of 3.5 μm. The maximum width was 0.35 μm near the distal end of the nucleus, and the minimum width was 0.17 μm near the apical end. Lamellar substructures were also present in the sperm nucleus, appearing at the periphery of the electron-dense cylinder in a radial manner. Staining with Feulgen's reaction and acid-orcein indicated that the coil was probably composed of sperm chromosomes.
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U2 - 10.1016/0014-4827(88)90282-0
DO - 10.1016/0014-4827(88)90282-0
M3 - Article
C2 - 3191949
AN - SCOPUS:0024261703
SN - 0014-4827
VL - 179
SP - 446
EP - 453
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -