A defective non-LTR retrotransposon is dispersed throughout the genome of the silkworm, Bombyx mori

Teru Ogura, Kazuhiro Okano, Kozo Tsuchida, Naoko Miyajima, Hideho Tanaka, Naoko Takada, Susumu Izumi, Shiro Tomino, Hideaki Maekawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The presence of long repetitive sequences is demonstrated in the genome of the silkworm, Bombyx mori. Members of this BMC1 family reveal several features typical of the L1 (long interspersed sequence one) family of mammals, except for species specific elements. The number of BMC1 elements is estimated to be approximately 3500 per haploid genome. Elements containing the full length unit of 5.1 kb are dispersed throughout the genome and their restriction sites are conserved, although most members are preferentially truncated to varying extents at their 5′ ends. DNA sequencing indicates that this element contains six tandem repeats of 15 bp CpG-rich sequence in the 5′ proximal region. It terminates with a 3′ oligo(A) stretch, and is flanked at both ends by a 7-10 bp target sequence duplication. In addition, there is significant evidence for amino acid sequence homology with reverse transcriptase domains of other L1 families, especially F, Doc and lockey of Drosophila melanogaster. No large open reading frame is present. The BMC1 element is suggested to be dispersed in the genome by a transposition mechanism involving RNA intermediates.

Original languageEnglish
Pages (from-to)311-323
Number of pages13
JournalChromosoma
Volume103
Issue number5
DOIs
Publication statusPublished - 1994 Sep
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Cite this

Ogura, T., Okano, K., Tsuchida, K., Miyajima, N., Tanaka, H., Takada, N., ... Maekawa, H. (1994). A defective non-LTR retrotransposon is dispersed throughout the genome of the silkworm, Bombyx mori. Chromosoma, 103(5), 311-323. https://doi.org/10.1007/BF00417878

A defective non-LTR retrotransposon is dispersed throughout the genome of the silkworm, Bombyx mori. / Ogura, Teru; Okano, Kazuhiro; Tsuchida, Kozo; Miyajima, Naoko; Tanaka, Hideho; Takada, Naoko; Izumi, Susumu; Tomino, Shiro; Maekawa, Hideaki.

In: Chromosoma, Vol. 103, No. 5, 09.1994, p. 311-323.

Research output: Contribution to journalArticle

Ogura, T, Okano, K, Tsuchida, K, Miyajima, N, Tanaka, H, Takada, N, Izumi, S, Tomino, S & Maekawa, H 1994, 'A defective non-LTR retrotransposon is dispersed throughout the genome of the silkworm, Bombyx mori', Chromosoma, vol. 103, no. 5, pp. 311-323. https://doi.org/10.1007/BF00417878
Ogura, Teru ; Okano, Kazuhiro ; Tsuchida, Kozo ; Miyajima, Naoko ; Tanaka, Hideho ; Takada, Naoko ; Izumi, Susumu ; Tomino, Shiro ; Maekawa, Hideaki. / A defective non-LTR retrotransposon is dispersed throughout the genome of the silkworm, Bombyx mori. In: Chromosoma. 1994 ; Vol. 103, No. 5. pp. 311-323.
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