A Disposable Sensor Chip Using a Paste Electrode with Surface-Imprinted Graphite Particles for Rapid and Reagentless Monitoring of Theophylline

Aaryashree ., Tomoji Ohishi, Yasuo Yoshimi

Research output: Contribution to journalArticlepeer-review

Abstract

This work focuses on a carbon-based imprinted polymer composite, employed as a molecular recognition and sensing interface in fabricating a disposable electrochemical sensor. The carbon-paste electrode was made of a molecularly imprinted polymer comprising a copolymer of methacrylic acid as the functional monomer and blended crosslinking monomers of N,N-methylenebisacrylamide, and ethylene glycol dimethacrylate, with theophylline as the template. The analytical properties of the proposed theophylline sensor were investigated, and the findings revealed an increase in differential pulse voltammetric current compared to the non-imprinted electrode. Under optimized conditions, the sensor has shown high sensitivity, high selectivity, lower detection limit (2.5 µg/mL), and satisfactory long-term stability. Further, the sensor was tested in whole bovine blood and vali-dated without any matrix effect and cross-reactivity. Additionally, chronoamperometry of the sensor chip supported a rapid determination of THO with a short response time of 3 s. This carbon-paste electrode is highly specific for theophylline and may be applied as a drug sensor for clinical use.

Original languageEnglish
Article number2456
JournalMolecules
Volume27
Issue number8
DOIs
Publication statusPublished - 2022 Apr 1

Keywords

  • carbon paste
  • disposable sensor
  • molecularly imprinted polymer
  • theophylline
  • therapeutic drug monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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