A Gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

Yoshitaka Nakayama, Kenjiro Yoshimura, Hidetoshi Iida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.

Original languageEnglish
Pages (from-to)5432-5434
Number of pages3
JournalApplied and Environmental Microbiology
Volume78
Issue number15
DOIs
Publication statusPublished - 2012 Aug
Externally publishedYes

Fingerprint

Corynebacterium glutamicum
secretion
glutamates
mutation
hysteresis
experiment
gain-of-function mutation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

A Gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion. / Nakayama, Yoshitaka; Yoshimura, Kenjiro; Iida, Hidetoshi.

In: Applied and Environmental Microbiology, Vol. 78, No. 15, 08.2012, p. 5432-5434.

Research output: Contribution to journalArticle

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