Abstract
A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.
| Original language | English |
|---|---|
| Pages (from-to) | 283-289 |
| Number of pages | 7 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 84 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 2002 May 15 |
| Externally published | Yes |
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Keywords
- Escherichia coli HB101
- ITO
- Microchannel
- PCR
- PS-Na
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
- Electrical and Electronic Engineering
Cite this
A heater-integrated transparent microchannel chip for continuous-flow PCR. / Sun, Kai; Yamaguchi, Akira; Ishida, Yutaka; Matsuo, Shigeki; Misawa, Hiroaki.
In: Sensors and Actuators, B: Chemical, Vol. 84, No. 2-3, 15.05.2002, p. 283-289.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A heater-integrated transparent microchannel chip for continuous-flow PCR
AU - Sun, Kai
AU - Yamaguchi, Akira
AU - Ishida, Yutaka
AU - Matsuo, Shigeki
AU - Misawa, Hiroaki
PY - 2002/5/15
Y1 - 2002/5/15
N2 - A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.
AB - A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.
KW - Escherichia coli HB101
KW - ITO
KW - Microchannel
KW - PCR
KW - PS-Na
UR - http://www.scopus.com/inward/record.url?scp=0037095347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037095347&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(02)00016-3
DO - 10.1016/S0925-4005(02)00016-3
M3 - Article
AN - SCOPUS:0037095347
VL - 84
SP - 283
EP - 289
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2-3
ER -