A heater-integrated transparent microchannel chip for continuous-flow PCR

Kai Sun, Akira Yamaguchi, Yutaka Ishida, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume84
Issue number2-3
DOIs
Publication statusPublished - 2002 May 15
Externally publishedYes

Fingerprint

polymerase chain reaction
Polymerase chain reaction
microchannels
Microchannels
heaters
chips
Tin oxides
indium oxides
Indium
tin oxides
Oxide films
oxide films
Quartz
Temperature distribution
temperature distribution
Substrates
temperature indicators
quartz
Glass
transparence

Keywords

  • Escherichia coli HB101
  • ITO
  • Microchannel
  • PCR
  • PS-Na

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

A heater-integrated transparent microchannel chip for continuous-flow PCR. / Sun, Kai; Yamaguchi, Akira; Ishida, Yutaka; Matsuo, Shigeki; Misawa, Hiroaki.

In: Sensors and Actuators, B: Chemical, Vol. 84, No. 2-3, 15.05.2002, p. 283-289.

Research output: Contribution to journalArticle

Sun, Kai ; Yamaguchi, Akira ; Ishida, Yutaka ; Matsuo, Shigeki ; Misawa, Hiroaki. / A heater-integrated transparent microchannel chip for continuous-flow PCR. In: Sensors and Actuators, B: Chemical. 2002 ; Vol. 84, No. 2-3. pp. 283-289.
@article{4e0a9dedd3044ce4b5f167ea58f831a5,
title = "A heater-integrated transparent microchannel chip for continuous-flow PCR",
abstract = "A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.",
keywords = "Escherichia coli HB101, ITO, Microchannel, PCR, PS-Na",
author = "Kai Sun and Akira Yamaguchi and Yutaka Ishida and Shigeki Matsuo and Hiroaki Misawa",
year = "2002",
month = "5",
day = "15",
doi = "10.1016/S0925-4005(02)00016-3",
language = "English",
volume = "84",
pages = "283--289",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - A heater-integrated transparent microchannel chip for continuous-flow PCR

AU - Sun, Kai

AU - Yamaguchi, Akira

AU - Ishida, Yutaka

AU - Matsuo, Shigeki

AU - Misawa, Hiroaki

PY - 2002/5/15

Y1 - 2002/5/15

N2 - A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.

AB - A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques and was sealed by another quartz glass substrate. Two indium-tin-oxide (ITO) films were deposited on the etched substrate as a thermal source. To confirm the temperature distribution in the microchannel, we measured the fluorescence spectra of an aqueous solution of 1-pyrenesulfonic acid sodium salt (PS-Na), which is a temperature-indicator dye, in the microchannel under a continuous solution flow. The results confirm that the temperature distribution on the microchannel's ITO films was almost uniform (within ±2 °C) under two flow rates (56 and 152 nl/min). The slightness of this deviation indicates that the ITO films integrated into the microchannel chip can be very useful as a thermal source for PCR. An amplification of a 450 bp segment of Escherichia coli HB101 was successfully performed by two-stage (94 and 67 °C) thermal cycling on the chip device.

KW - Escherichia coli HB101

KW - ITO

KW - Microchannel

KW - PCR

KW - PS-Na

UR - http://www.scopus.com/inward/record.url?scp=0037095347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037095347&partnerID=8YFLogxK

U2 - 10.1016/S0925-4005(02)00016-3

DO - 10.1016/S0925-4005(02)00016-3

M3 - Article

VL - 84

SP - 283

EP - 289

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 2-3

ER -