A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto; Keita Hamasaki; Hisakazu Mihara; Akihiko Ueno

doi:10.1016/S0960-894X(00)00359-0

A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto, Keita Hamasaki, Hisakazu Mihara, Akihiko Ueno

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

A 16-residue peptide, including the Tat_49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	1857-1861
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	10
Issue number	16
DOIs	https://doi.org/10.1016/S0960-894X(00)00359-0
Publication status	Published - 2000 Aug 21
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. / Matsumoto, Chiharu; Hamasaki, Keita; Mihara, Hisakazu; Ueno, Akihiko.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 10, No. 16, 21.08.2000, p. 1857-1861.

Research output: Contribution to journal › Article › peer-review

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abstract = "A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.",

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AU - Ueno, Akihiko

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A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Abstract

ASJC Scopus subject areas

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