A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto, Keita Hamasaki, Hisakazu Mihara, Akihiko Ueno

Research output: Contribution to journal › Article

44 Citations (Scopus)

Abstract

A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1857-1861
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume10
Issue number16
DOIs
Publication statusPublished - 2000 Aug 21
Externally publishedYes

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Screening
Throughput
RNA
Peptides
Molecules
Fluorescein
Coloring Agents
Pharmaceutical Preparations
Fluorescence Resonance Energy Transfer
tetramethylrhodamine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. / Matsumoto, Chiharu; Hamasaki, Keita; Mihara, Hisakazu; Ueno, Akihiko.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 10, No. 16, 21.08.2000, p. 1857-1861.

Research output: Contribution to journal › Article

Matsumoto, C, Hamasaki, K, Mihara, H & Ueno, A 2000, 'A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically', Bioorganic and Medicinal Chemistry Letters, vol. 10, no. 16, pp. 1857-1861. https://doi.org/10.1016/S0960-894X(00)00359-0
Matsumoto C, Hamasaki K, Mihara H, Ueno A. A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. Bioorganic and Medicinal Chemistry Letters. 2000 Aug 21;10(16):1857-1861. https://doi.org/10.1016/S0960-894X(00)00359-0
Matsumoto, Chiharu ; Hamasaki, Keita ; Mihara, Hisakazu ; Ueno, Akihiko. / A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. In: Bioorganic and Medicinal Chemistry Letters. 2000 ; Vol. 10, No. 16. pp. 1857-1861.
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