A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto, Keita Hamasaki, Hisakazu Mihara, Akihiko Ueno

Research output: Contribution to journal › Article

44 Citations (Scopus)

Abstract

A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1857-1861
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume10
Issue number16
DOIs
Publication statusPublished - 2000 Aug 21
Externally publishedYes

Fingerprint

Screening
Throughput
RNA
Peptides
Molecules
Fluorescein
Coloring Agents
Pharmaceutical Preparations
Fluorescence Resonance Energy Transfer
tetramethylrhodamine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. / Matsumoto, Chiharu; Hamasaki, Keita; Mihara, Hisakazu; Ueno, Akihiko.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 10, No. 16, 21.08.2000, p. 1857-1861.

Research output: Contribution to journal › Article

Matsumoto, C, Hamasaki, K, Mihara, H & Ueno, A 2000, 'A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically', Bioorganic and Medicinal Chemistry Letters, vol. 10, no. 16, pp. 1857-1861. https://doi.org/10.1016/S0960-894X(00)00359-0
Matsumoto C, Hamasaki K, Mihara H, Ueno A. A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. Bioorganic and Medicinal Chemistry Letters. 2000 Aug 21;10(16):1857-1861. https://doi.org/10.1016/S0960-894X(00)00359-0
Matsumoto, Chiharu ; Hamasaki, Keita ; Mihara, Hisakazu ; Ueno, Akihiko. / A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically. In: Bioorganic and Medicinal Chemistry Letters. 2000 ; Vol. 10, No. 16. pp. 1857-1861.
@article{746b605348b642098e34784d3b0c8f8f,
title = "A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically",
abstract = "A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.",
author = "Chiharu Matsumoto and Keita Hamasaki and Hisakazu Mihara and Akihiko Ueno",
year = "2000",
month = "8",
day = "21",
doi = "10.1016/S0960-894X(00)00359-0",
language = "English",
volume = "10",
pages = "1857--1861",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",
number = "16",

}

TY - JOUR

T1 - A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

AU - Matsumoto, Chiharu

AU - Hamasaki, Keita

AU - Mihara, Hisakazu

AU - Ueno, Akihiko

PY - 2000/8/21

Y1 - 2000/8/21

N2 - A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

AB - A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

UR - http://www.scopus.com/inward/record.url?scp=0034698782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034698782&partnerID=8YFLogxK

U2 - 10.1016/S0960-894X(00)00359-0

DO - 10.1016/S0960-894X(00)00359-0

M3 - Article

VL - 10

SP - 1857

EP - 1861

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 16

ER -

Access to Document