Abstract
Hybrid small-diameter tubes were fabricated by wrapping decellularized aortic intima-media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima-media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima-media with electrospun fibers generates a small-diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties.
| Original language | English |
|---|---|
| Journal | Journal of Biomedical Materials Research - Part A |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
Fingerprint
Keywords
- decellularized aortic intima-media
- electrospinning
- mechanical compliance
- small-diameter blood vessel
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys
Cite this
A hybrid small-diameter tube fabricated from decellularized aortic intima-media and electrospun fiber for artificial small-diameter blood vessel. / Wu, Pingli; Nakamura, Naoko; Morita, Hiroko; Nam, Kwangwoo; Fujisato, Toshiya; Kimura, Tsuyoshi; Kishida, Akio.
In: Journal of Biomedical Materials Research - Part A, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A hybrid small-diameter tube fabricated from decellularized aortic intima-media and electrospun fiber for artificial small-diameter blood vessel
AU - Wu, Pingli
AU - Nakamura, Naoko
AU - Morita, Hiroko
AU - Nam, Kwangwoo
AU - Fujisato, Toshiya
AU - Kimura, Tsuyoshi
AU - Kishida, Akio
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Hybrid small-diameter tubes were fabricated by wrapping decellularized aortic intima-media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima-media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima-media with electrospun fibers generates a small-diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties.
AB - Hybrid small-diameter tubes were fabricated by wrapping decellularized aortic intima-media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima-media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima-media with electrospun fibers generates a small-diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties.
KW - decellularized aortic intima-media
KW - electrospinning
KW - mechanical compliance
KW - small-diameter blood vessel
UR - http://www.scopus.com/inward/record.url?scp=85061935421&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061935421&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.36631
DO - 10.1002/jbm.a.36631
M3 - Article
C2 - 30698329
AN - SCOPUS:85061935421
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
ER -