TY - JOUR
T1 - A hybrid small-diameter tube fabricated from decellularized aortic intima-media and electrospun fiber for artificial small-diameter blood vessel
AU - Wu, Pingli
AU - Nakamura, Naoko
AU - Morita, Hiroko
AU - Nam, Kwangwoo
AU - Fujisato, Toshiya
AU - Kimura, Tsuyoshi
AU - Kishida, Akio
N1 - Funding Information:
Additional Supporting Information may be found in the online version of this article. †Present address: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, People’s Republic of China ††Present address: Department of Bioscience and Engineering, Shibaura Institute of Technology, Saitama, Japan Correspondence to: T. Kimura; e-mail: kimurat.mbme@tmd.ac.jp Contract grant sponsor: Japan Society for the Promotion of Science; contract grant number: KAKENHI 16H03180, 16H03181 Contract grant sponsor: Japanese Society for Artificial Organs; contract grant number: 2015 Yoshimi Memorial T. M. P grant Contract grant sponsor: Ministry of Education, Culture, Sports, Science and Technology; contract grant number: Cooperative Research Project of Research Center and Creative Scientific Research of the Viable Materia Contract grant sponsor: China Postdoctoral Science Foundation; contract grant number: 2017M620090 Contract grant sponsor: National Natural Science Foundation of China; contract grant number: 31700845 Contract grant sponsor: JSPS, 2015 Yoshimi Memorial T. M. P grant; contract grant number: 16H03181 and 16H03180
Funding Information:
This work was partly supported by a Grant-in-Aid for Scientific Research (B) (16H03180, 16H03181) from JSPS, 2015 Yoshimi Memorial T. M. P grant, and the Cooperative Research Project of Research Center for Biomedical Engineering and Creative Scientific Research of the Viable Material via Integration of Biology and Engineering from MEXT. The authors thank Dr. Takehisa Matsuda for providing the electrospinning equipment. P. Wu would like to acknowledge the financial support from the National Natural Science Foundation of China (31700845), China Postdoctoral Science Foundation (2017M620090).
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2019/5
Y1 - 2019/5
N2 - Hybrid small-diameter tubes were fabricated by wrapping decellularized aortic intima-media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima-media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima-media with electrospun fibers generates a small-diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties.
AB - Hybrid small-diameter tubes were fabricated by wrapping decellularized aortic intima-media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima-media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima-media with electrospun fibers generates a small-diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties.
KW - decellularized aortic intima-media
KW - electrospinning
KW - mechanical compliance
KW - small-diameter blood vessel
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U2 - 10.1002/jbm.a.36631
DO - 10.1002/jbm.a.36631
M3 - Article
C2 - 30698329
AN - SCOPUS:85061935421
VL - 107
SP - 1064
EP - 1070
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 5
ER -