A hybrid small-diameter tube fabricated from decellularized aortic intima-media and electrospun fiber for artificial small-diameter blood vessel

Pingli Wu, Naoko Nakamura, Hiroko Morita, Kwangwoo Nam, Toshiya Fujisato, Tsuyoshi Kimura, Akio Kishida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hybrid small-diameter tubes were fabricated by wrapping decellularized aortic intima-media sheets around a tubular stainless steel mandrel with diameter 4 mm, and then by coating with electrospun segmented polyurethane. The synthetic coat was deposited uniformly to a thickness of about 0.5–3.5 μm depending on the duration of electrospinning. Resistance to luminal pressure, burst strength, and stiffness increased with the thickness of the electrospun coat, suggesting that the synthetic fabric reinforces the reconstructed acellular aortic intima-media. Human umbilical vein endothelial cells seeded on the inner surface acquired flagstone morphology, while normal human dermal fibroblasts seeded on the outer surface proliferated well and partly migrated into deeper layers. Collectively, the data suggest that reinforcing decellularized aortic intima-media with electrospun fibers generates a small-diameter hybrid blood vessel with good biocompatibility and suitable mechanical properties.

Original languageEnglish
JournalJournal of Biomedical Materials Research - Part A
DOIs
Publication statusPublished - 2019 Jan 1

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Keywords

  • decellularized aortic intima-media
  • electrospinning
  • mechanical compliance
  • small-diameter blood vessel

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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