A Klebsiella aerogenes moaEF operon is controlled by the positive MoaR regulator of the monoamine regulon

Hiroyuki Azakami, Hiroyuki Sugino, Nobuhide Iwata, Natsuko Yokoro, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A 30-kDa protein accumulated upon induction by a high concentration of tyramine or dopamine in cells of Klebsiella aerogenes (Ka). These cells carried a plasmid (pAS123) that included the arylsulfatase operon (atsBA). Deletion analysis showed that the region essential for induction of the 30-kDa protein was located within a 2.0-kb cloned segment downstream of the atsBA operon. The nucleotide (nt) sequence of the 2.0-kb fragment revealed two open reading frames (ORFs), moaE and moaF. Transcription from a putative promoter of moaE was induced by the addition of tyramine, and the moaF gene was co-transcribed from this monoamine-inducible Ka promoter. The deduced Ka MoaE protein was homologous to insect-type alcohol dehydrogenase. The sequence of the 18 amino acids from the N-terminus of the purified 30-kDa protein agreed with that deduced from the nt sequence of moaF. Using a Ka strain with a mutant moaR gene, we found that MoaR, that acts as the positive regulator of the monoamine regulon, also acts as the positive regulator of the moaEF operon.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalGene
Volume164
Issue number1
DOIs
Publication statusPublished - 1995 Oct 16
Externally publishedYes

Keywords

  • catecholamine metabolism
  • DNA sequence
  • dopamine
  • transcriptional regulation
  • Tyramine

ASJC Scopus subject areas

  • Genetics

Cite this

A Klebsiella aerogenes moaEF operon is controlled by the positive MoaR regulator of the monoamine regulon. / Azakami, Hiroyuki; Sugino, Hiroyuki; Iwata, Nobuhide; Yokoro, Natsuko; Yamashita, Mitsuo; Murooka, Yoshikatsu.

In: Gene, Vol. 164, No. 1, 16.10.1995, p. 89-94.

Research output: Contribution to journalArticle

Azakami, Hiroyuki ; Sugino, Hiroyuki ; Iwata, Nobuhide ; Yokoro, Natsuko ; Yamashita, Mitsuo ; Murooka, Yoshikatsu. / A Klebsiella aerogenes moaEF operon is controlled by the positive MoaR regulator of the monoamine regulon. In: Gene. 1995 ; Vol. 164, No. 1. pp. 89-94.
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