A new reagentless immunosensor for measuring IgG concentration in human plasma based on fluorescence-enhancement immunoassay

S. Aoyagi, T. Miyasaka, Yasuo Yoshimi, K. Sakai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Based on the enhancement of fluorescein isothiocyanate (FITC) fluorescence caused by binding to proteins, we developed a reagentless immunosensor to directly determine immunoglobulin G (IgG) in human plasma. The fluorescence-enhancement immunoassay (FEI) is used in only limited conditions in spite of its simplicity, because the homogeneous reagent cannot be reused. In the present study, a new FEI was evaluated with an immobilized reagent. FITC was used to label protein A, which has specific reactivity with IgG. The FITC-labeled protein A was immobilized on nylon membrane and placed in a fluorometer cell. It produced fluorescence enhancement corresponding to IgG concentration in phosphate-buffered saline (PBS) and bovine serum solution, and it responded to IgG also in human plasma. Thus the IgG concentration can be determined directly using the reagentless immunosensor based on FEI.

Original languageEnglish
Pages (from-to)60-63
Number of pages4
JournalJournal of Artificial Organs
Volume5
Issue number1
Publication statusPublished - 2002

Fingerprint

Plasma (human)
Immunosensors
Immunoglobulin G
Fluorescence
Fluorescein
Staphylococcal Protein A
Proteins
Fluorometers
Nylons
Labels
Phosphates
Membranes
isothiocyanic acid

Keywords

  • FITC
  • Fluorescence-enhancement immunoassay
  • IgG
  • Protein A
  • Reagentless immunosensor

ASJC Scopus subject areas

  • Biophysics

Cite this

A new reagentless immunosensor for measuring IgG concentration in human plasma based on fluorescence-enhancement immunoassay. / Aoyagi, S.; Miyasaka, T.; Yoshimi, Yasuo; Sakai, K.

In: Journal of Artificial Organs, Vol. 5, No. 1, 2002, p. 60-63.

Research output: Contribution to journalArticle

@article{c8084ccbddfd44cfa0e5b5ebb2f33177,
title = "A new reagentless immunosensor for measuring IgG concentration in human plasma based on fluorescence-enhancement immunoassay",
abstract = "Based on the enhancement of fluorescein isothiocyanate (FITC) fluorescence caused by binding to proteins, we developed a reagentless immunosensor to directly determine immunoglobulin G (IgG) in human plasma. The fluorescence-enhancement immunoassay (FEI) is used in only limited conditions in spite of its simplicity, because the homogeneous reagent cannot be reused. In the present study, a new FEI was evaluated with an immobilized reagent. FITC was used to label protein A, which has specific reactivity with IgG. The FITC-labeled protein A was immobilized on nylon membrane and placed in a fluorometer cell. It produced fluorescence enhancement corresponding to IgG concentration in phosphate-buffered saline (PBS) and bovine serum solution, and it responded to IgG also in human plasma. Thus the IgG concentration can be determined directly using the reagentless immunosensor based on FEI.",
keywords = "FITC, Fluorescence-enhancement immunoassay, IgG, Protein A, Reagentless immunosensor",
author = "S. Aoyagi and T. Miyasaka and Yasuo Yoshimi and K. Sakai",
year = "2002",
language = "English",
volume = "5",
pages = "60--63",
journal = "Journal of Artificial Organs",
issn = "1434-7229",
publisher = "Springer Japan",
number = "1",

}

TY - JOUR

T1 - A new reagentless immunosensor for measuring IgG concentration in human plasma based on fluorescence-enhancement immunoassay

AU - Aoyagi, S.

AU - Miyasaka, T.

AU - Yoshimi, Yasuo

AU - Sakai, K.

PY - 2002

Y1 - 2002

N2 - Based on the enhancement of fluorescein isothiocyanate (FITC) fluorescence caused by binding to proteins, we developed a reagentless immunosensor to directly determine immunoglobulin G (IgG) in human plasma. The fluorescence-enhancement immunoassay (FEI) is used in only limited conditions in spite of its simplicity, because the homogeneous reagent cannot be reused. In the present study, a new FEI was evaluated with an immobilized reagent. FITC was used to label protein A, which has specific reactivity with IgG. The FITC-labeled protein A was immobilized on nylon membrane and placed in a fluorometer cell. It produced fluorescence enhancement corresponding to IgG concentration in phosphate-buffered saline (PBS) and bovine serum solution, and it responded to IgG also in human plasma. Thus the IgG concentration can be determined directly using the reagentless immunosensor based on FEI.

AB - Based on the enhancement of fluorescein isothiocyanate (FITC) fluorescence caused by binding to proteins, we developed a reagentless immunosensor to directly determine immunoglobulin G (IgG) in human plasma. The fluorescence-enhancement immunoassay (FEI) is used in only limited conditions in spite of its simplicity, because the homogeneous reagent cannot be reused. In the present study, a new FEI was evaluated with an immobilized reagent. FITC was used to label protein A, which has specific reactivity with IgG. The FITC-labeled protein A was immobilized on nylon membrane and placed in a fluorometer cell. It produced fluorescence enhancement corresponding to IgG concentration in phosphate-buffered saline (PBS) and bovine serum solution, and it responded to IgG also in human plasma. Thus the IgG concentration can be determined directly using the reagentless immunosensor based on FEI.

KW - FITC

KW - Fluorescence-enhancement immunoassay

KW - IgG

KW - Protein A

KW - Reagentless immunosensor

UR - http://www.scopus.com/inward/record.url?scp=0036214593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036214593&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 60

EP - 63

JO - Journal of Artificial Organs

JF - Journal of Artificial Organs

SN - 1434-7229

IS - 1

ER -