A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia

Rutchadaporn Sriprang, Makoto Hayashi, Mitsuo Yamashita, Hisayo Ono, Kazuhiko Saeki, Yoshikatsu Murooka

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A novel plant-bacterial remediation system for heavy metals (HM) was developed by expression of tetrameric human metallothionein (MTL4) in Mesorhizobium huakuii subsp. rengei B3, a strain which infects and forms nodules on a green manure, Astragalus sinicus. The MTL4 gene was fused to the nifH and nolB promoters, which generated nodule- specific expression of the MTL4 gene. The expression analysis of the MTL4 gene was demonstrated in free-living cells in the presence of Cd2+ and Cu2+, under the low oxygen condition. The MTL4 under the nifH and nolB promoters was expressed and increased the accumulation of Cd2+, but not Cu2+ in free-living cells. The expression of the integrated nifH-MTL4 gene in the chromosome of strain B3 was also expressed stably and accumulated Cd2+ in the bacterial cells. The MTL4 transcripts were detected by in situ hybridization in bacteroids of mature nodules of A. sinicus containing nifH-MTL4 and nolB-MTL4 fusion gene. Moreover the MTL4 protein was detected by immunostaining. By infection of the recombinant B3, A. sinicus established symbiosis with the recombinant B3 that was grown in Cd2+ and Cu2+-polluted soils. The symbionts increased Cd2+ accumulation in nodules 1.7-2.0-fold, whereas, no significantly increase in Cu2+ accumulation was noted.

Original languageEnglish
Pages (from-to)279-293
Number of pages15
JournalJournal of Biotechnology
Volume99
Issue number3
DOIs
Publication statusPublished - 2002 Nov 13
Externally publishedYes

Fingerprint

Bioremediation
Heavy Metals
Heavy metals
Genes
Cells
Metallothionein
Manures
Chromosomes
Remediation
Fusion reactions
Oxygen
Proteins
Soils

Keywords

  • Astragalus sinicus
  • Heavy metal
  • Mesorhizobium huakuii
  • Metallothionein
  • Symbiosis
  • Symbiotic engineering

ASJC Scopus subject areas

  • Biotechnology

Cite this

A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia. / Sriprang, Rutchadaporn; Hayashi, Makoto; Yamashita, Mitsuo; Ono, Hisayo; Saeki, Kazuhiko; Murooka, Yoshikatsu.

In: Journal of Biotechnology, Vol. 99, No. 3, 13.11.2002, p. 279-293.

Research output: Contribution to journalArticle

Sriprang, Rutchadaporn ; Hayashi, Makoto ; Yamashita, Mitsuo ; Ono, Hisayo ; Saeki, Kazuhiko ; Murooka, Yoshikatsu. / A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia. In: Journal of Biotechnology. 2002 ; Vol. 99, No. 3. pp. 279-293.
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