A novel hairless mouse model for malignant melanoma

Nguyen Dinh Thang, Ichiro Yajima, Kaoru Nakagawa, Toyonori Tsuzuki, Mayuko Y. Kumasaka, Nobutaka Ohgami, Thuy B. Ly, Takashi Iwamoto, Daisuke Watanabe, Masashi Kato

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9 Citations (Scopus)

Abstract

Background: An appropriate animal model for malignant melanoma could be a strong tool to develop biomarkers through analysis of melanomagenesis. Objective: Development of a novel animal model that spontaneously develops malignant melanoma with a high percentage. Methods: We crossed oncogenic RET (RFP-RET)-carrying transgenic mice of line 304/B6 (RET-mice) with hairless mice (hr/hr) and newly established hairless RFP-RET-transgenic mice of line 304-hr/hr (HL-RET-mice). Results: The HL-RET-mice developed hyperpigmented skin and benign melanocytic tumors without exception. More importantly, 63.8% (46/72) of the benign tumors were transformed to malignant melanoma in the HL-RET-mice. Mean time until the development of benign melanocytic tumors (2.4 months; n= 102) in the HL-RET-mice was about half of that in the original RET-mice (4.6 months; n= 20). Mean life span in the HL-RET-mice (9.7 months; n= 38) was also significantly (p< 0.01) shorter than that in the original RET-mice (10.8 months; n= 20). Since early development of tumors could contribute to shortening of the research period, HL-RET-mice could be a useful model for analysis of melanomagenesis. We then found that the expression level of Mps one binder kinase activator-like-2B (Mobkl2b) in benign tumors was higher than that in malignant melanoma in HL-RET-mice. Expression level of MOBKL2B in malignant melanoma cell lines was also lower than that in non-malignant melanocytic cells in mice and humans, suggesting that MOBKL2B could be a novel marker for malignant melanoma. Conclusion: We established a novel hairless RET-transgenic mouse line spontaneously developing cutaneous malignant melanomas from benign melanocytic tumors. This mouse model may be useful to find new candidates of melanoma-related molecule.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalJournal of Dermatological Science
Volume65
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

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Keywords

  • Animal model
  • Hairlessness
  • MOBKL2B
  • Melanoma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Thang, N. D., Yajima, I., Nakagawa, K., Tsuzuki, T., Kumasaka, M. Y., Ohgami, N., Ly, T. B., Iwamoto, T., Watanabe, D., & Kato, M. (2012). A novel hairless mouse model for malignant melanoma. Journal of Dermatological Science, 65(3), 207-212. https://doi.org/10.1016/j.jdermsci.2011.10.010