A novel method of immobilizing antibodies on a quartz crystal microbalance using plasma-polymerized films for immunosensors

Keijiro Nakanishi, Hitoshi Muguruma, Isao Karube

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

A novel method of immobilizing antibodies on quartz crystals for use in immunosensors was developed using an ethylenediamine plasma-polymerized film matrix. The films formed on the quartz crystals are extremely thin and homogeneous, and they incorporate amino groups. Sensors produced using this method are more reproducible from sample to sample and exhibit lower noise and higher sensitivity than sensors made using conventional immobilization methods, e.g., via polyethylenimine and (γ-aminopropyl)trimethoxysilane. This is, to our knowledge, the first reported application of plasma-polymerized films to quartz crystal microbalance immunosensors. Results on orientation-controlled immobilization of antibodies, reusability and calibration tests are also presented.

Original languageEnglish
Pages (from-to)1695-1700
Number of pages6
JournalAnalytical Chemistry
Volume68
Issue number10
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Immunosensors
Quartz crystal microbalances
ethylenediamine
Quartz
Plasmas
Antibodies
Polyethyleneimine
Crystals
Sensors
Reusability
Calibration

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

A novel method of immobilizing antibodies on a quartz crystal microbalance using plasma-polymerized films for immunosensors. / Nakanishi, Keijiro; Muguruma, Hitoshi; Karube, Isao.

In: Analytical Chemistry, Vol. 68, No. 10, 1996, p. 1695-1700.

Research output: Contribution to journalArticle

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