Abstract
A novel method of immobilizing antibodies on quartz crystals for use in immunosensors was developed using an ethylenediamine plasma-polymerized film matrix. The films formed on the quartz crystals are extremely thin and homogeneous, and they incorporate amino groups. Sensors produced using this method are more reproducible from sample to sample and exhibit lower noise and higher sensitivity than sensors made using conventional immobilization methods, e.g., via polyethylenimine and (γ-aminopropyl)trimethoxysilane. This is, to our knowledge, the first reported application of plasma-polymerized films to quartz crystal microbalance immunosensors. Results on orientation-controlled immobilization of antibodies, reusability and calibration tests are also presented.
| Original language | English |
|---|---|
| Pages (from-to) | 1695-1700 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 68 |
| Issue number | 10 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
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A novel method of immobilizing antibodies on a quartz crystal microbalance using plasma-polymerized films for immunosensors. / Nakanishi, Keijiro; Muguruma, Hitoshi; Karube, Isao.
In: Analytical Chemistry, Vol. 68, No. 10, 1996, p. 1695-1700.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel method of immobilizing antibodies on a quartz crystal microbalance using plasma-polymerized films for immunosensors
AU - Nakanishi, Keijiro
AU - Muguruma, Hitoshi
AU - Karube, Isao
PY - 1996
Y1 - 1996
N2 - A novel method of immobilizing antibodies on quartz crystals for use in immunosensors was developed using an ethylenediamine plasma-polymerized film matrix. The films formed on the quartz crystals are extremely thin and homogeneous, and they incorporate amino groups. Sensors produced using this method are more reproducible from sample to sample and exhibit lower noise and higher sensitivity than sensors made using conventional immobilization methods, e.g., via polyethylenimine and (γ-aminopropyl)trimethoxysilane. This is, to our knowledge, the first reported application of plasma-polymerized films to quartz crystal microbalance immunosensors. Results on orientation-controlled immobilization of antibodies, reusability and calibration tests are also presented.
AB - A novel method of immobilizing antibodies on quartz crystals for use in immunosensors was developed using an ethylenediamine plasma-polymerized film matrix. The films formed on the quartz crystals are extremely thin and homogeneous, and they incorporate amino groups. Sensors produced using this method are more reproducible from sample to sample and exhibit lower noise and higher sensitivity than sensors made using conventional immobilization methods, e.g., via polyethylenimine and (γ-aminopropyl)trimethoxysilane. This is, to our knowledge, the first reported application of plasma-polymerized films to quartz crystal microbalance immunosensors. Results on orientation-controlled immobilization of antibodies, reusability and calibration tests are also presented.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030142226&partnerID=8YFLogxK
M3 - Article
C2 - 8651481
AN - SCOPUS:0030142226
VL - 68
SP - 1695
EP - 1700
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 10
ER -