A novel Xenopus laevis SRY-related gene, xSox33

Jun Hagiuda, Yoshiki Hiraoka, Masanori Hasegawa, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1628
Issue number2
DOIs
Publication statusPublished - 2003 Jul 28
Externally publishedYes

Keywords

  • (Xenopus laevis)
  • DNA binding
  • Embryogenesis
  • HMG box
  • SOX
  • SRY

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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