A photoresponsive polymer surface which enables in situ control of living cell adhesion

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Katsuhide Ohi, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2339
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CityYokohama
Period05/5/2505/5/27

Fingerprint

Cell adhesion
Polymers
Chromophores
Cell culture
Washing
Linewidth
Cells
Irradiation
Temperature

Keywords

  • Cell adhesion control
  • Living cell
  • Photoresponsive culture surface

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Edahiro, J. I., Sumaru, K., Tada, Y., Ohi, K., Takagi, T., Shinbo, T., ... Yoshimi, Y. (2005). A photoresponsive polymer surface which enables in situ control of living cell adhesion. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2339)

A photoresponsive polymer surface which enables in situ control of living cell adhesion. / Edahiro, Jun Ichi; Sumaru, Kimio; Tada, Yuichi; Ohi, Katsuhide; Takagi, Toshiyuki; Shinbo, Toshio; Kanamori, Toshiyuki; Yoshimi, Yasuo.

Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2339.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Edahiro, JI, Sumaru, K, Tada, Y, Ohi, K, Takagi, T, Shinbo, T, Kanamori, T & Yoshimi, Y 2005, A photoresponsive polymer surface which enables in situ control of living cell adhesion. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2339, 54th SPSJ Annual Meeting 2005, Yokohama, 05/5/25.
Edahiro JI, Sumaru K, Tada Y, Ohi K, Takagi T, Shinbo T et al. A photoresponsive polymer surface which enables in situ control of living cell adhesion. In Polymer Preprints, Japan. 1 ed. Vol. 54. 2005. p. 2339
Edahiro, Jun Ichi ; Sumaru, Kimio ; Tada, Yuichi ; Ohi, Katsuhide ; Takagi, Toshiyuki ; Shinbo, Toshio ; Kanamori, Toshiyuki ; Yoshimi, Yasuo. / A photoresponsive polymer surface which enables in situ control of living cell adhesion. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2339
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