A photoresponsive polymer surface which enables in situ control of living cell adhesion

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Katsuhide Ohi, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Contribution to conferencePaper

Abstract

Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Conference

Conference54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Cell adhesion control
  • Living cell
  • Photoresponsive culture surface

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'A photoresponsive polymer surface which enables in situ control of living cell adhesion'. Together they form a unique fingerprint.

  • Cite this

    Edahiro, J. I., Sumaru, K., Tada, Y., Ohi, K., Takagi, T., Shinbo, T., Kanamori, T., & Yoshimi, Y. (2005). A photoresponsive polymer surface which enables in situ control of living cell adhesion. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.