A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Contribution to conferencePaper

Abstract

We have been developing a novel "on-demand cell manipulation technique" which enables control of cell adhesion by regional photo-irradiation on adhering region of objective cells. In this study, our objective is to improve performance of the "photoresponsive culture surface (PRCS) " that can control cell adhesion by photo-irradiation and temperature change and that is the key material for our novel technique. Firstly, cell adhesion control of extended cells was successfully achieved for the first time. PRCS was prepared with pory(N-isopropylacrylamide) containing photochromic chromophore, 6-nitrospiropyran. On this PRCS, MDCK cells were attached and extended evenly as a sheet after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) and cooled at 15°C. Then the PRCS was washed by fresh medium to remove weakly adhering cells in non-irradiated region. As a result, we confirmed that this method was applicable even to extended cells. Additionally, co-culture system was produced in simple procedures by culturing another kind of cells (CHO-K1 cells) in the cell-detached area. Secondary, for lowering cell damage from low temperature treatment, we prepared another type of PRCS with poly(N,N-dimethylacrylamide) containing 6-nitrospiropyran, which has no thermoresponsive nature. We confirmed that adhesion of MDCK cells on this novel PRCS could be changed only by photo-irradiation without any temperature change. Additionally, this result also supports our hypothesis that spiropyran chromophore directly captures cells in cell adhesion control.

Original languageEnglish
Pages5213-5214
Number of pages2
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Conference

Conference54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

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Keywords

  • Cell adhesion control
  • On-demand cell manipulation
  • Photoresponsive culture surface
  • Photoresponsive polymer
  • Spiropyran. N-isopropylacrylamide

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Edahiro, J. I., Sumaru, K., Tada, Y., Takagi, T., Shinbo, T., Kanamori, T., & Yoshimi, Y. (2005). A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. 5213-5214. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.