A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have been developing a novel "on-demand cell manipulation technique" which enables control of cell adhesion by regional photo-irradiation on adhering region of objective cells. In this study, our objective is to improve performance of the "photoresponsive culture surface (PRCS) " that can control cell adhesion by photo-irradiation and temperature change and that is the key material for our novel technique. Firstly, cell adhesion control of extended cells was successfully achieved for the first time. PRCS was prepared with pory(N-isopropylacrylamide) containing photochromic chromophore, 6-nitrospiropyran. On this PRCS, MDCK cells were attached and extended evenly as a sheet after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) and cooled at 15°C. Then the PRCS was washed by fresh medium to remove weakly adhering cells in non-irradiated region. As a result, we confirmed that this method was applicable even to extended cells. Additionally, co-culture system was produced in simple procedures by culturing another kind of cells (CHO-K1 cells) in the cell-detached area. Secondary, for lowering cell damage from low temperature treatment, we prepared another type of PRCS with poly(N,N-dimethylacrylamide) containing 6-nitrospiropyran, which has no thermoresponsive nature. We confirmed that adhesion of MDCK cells on this novel PRCS could be changed only by photo-irradiation without any temperature change. Additionally, this result also supports our hypothesis that spiropyran chromophore directly captures cells in cell adhesion control.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages5213-5214
Number of pages2
Volume54
Edition2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CityYamagata
Period05/9/2005/9/22

Fingerprint

Adhesives
Cell adhesion
Polymers
Irradiation
Chromophores
Cell culture
Temperature
Adhesion
Cells

Keywords

  • Cell adhesion control
  • On-demand cell manipulation
  • Photoresponsive culture surface
  • Photoresponsive polymer
  • Spiropyran. N-isopropylacrylamide

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Edahiro, J. I., Sumaru, K., Tada, Y., Takagi, T., Shinbo, T., Kanamori, T., & Yoshimi, Y. (2005). A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. In Polymer Preprints, Japan (2 ed., Vol. 54, pp. 5213-5214)

A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. / Edahiro, Jun Ichi; Sumaru, Kimio; Tada, Yuichi; Takagi, Toshiyuki; Shinbo, Toshio; Kanamori, Toshiyuki; Yoshimi, Yasuo.

Polymer Preprints, Japan. Vol. 54 2. ed. 2005. p. 5213-5214.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Edahiro, JI, Sumaru, K, Tada, Y, Takagi, T, Shinbo, T, Kanamori, T & Yoshimi, Y 2005, A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. in Polymer Preprints, Japan. 2 edn, vol. 54, pp. 5213-5214, 54th SPSJ Symposium on Macromolecules, Yamagata, 05/9/20.
Edahiro JI, Sumaru K, Tada Y, Takagi T, Shinbo T, Kanamori T et al. A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. In Polymer Preprints, Japan. 2 ed. Vol. 54. 2005. p. 5213-5214
Edahiro, Jun Ichi ; Sumaru, Kimio ; Tada, Yuichi ; Takagi, Toshiyuki ; Shinbo, Toshio ; Kanamori, Toshiyuki ; Yoshimi, Yasuo. / A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. Polymer Preprints, Japan. Vol. 54 2. ed. 2005. pp. 5213-5214
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