A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing

Runa Nakamura, Hitoshi Muguruma, Kazunori Ikebukuro, Satoshi Sasaki, Ryohei Nagata, Isao Karube, Henrik Pedersen

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Abstract

We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed on the gold surfaces are extremely thin (∼100 nm), are homogeneous, demonstrate good adhesion, have a flat profile, and incorporate amino groups to introduce a chemical functionality. A sensor chip made with the film has many advantages when compared with conventionally used designs such as carboxylated dextran hydrogels. For example, a sensor chip made with the film shows a better sensor response than a conventional design partly because antibodies are densely and two-dimensionally immobilized onto the surface of the plasma-polymerized film.

Original languageEnglish
Pages (from-to)4649-4652
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number22
Publication statusPublished - 1997 Nov 15
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Nakamura, R., Muguruma, H., Ikebukuro, K., Sasaki, S., Nagata, R., Karube, I., & Pedersen, H. (1997). A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing. Analytical Chemistry, 69(22), 4649-4652.