Abstract
We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed on the gold surfaces are extremely thin (∼100 nm), are homogeneous, demonstrate good adhesion, have a flat profile, and incorporate amino groups to introduce a chemical functionality. A sensor chip made with the film has many advantages when compared with conventionally used designs such as carboxylated dextran hydrogels. For example, a sensor chip made with the film shows a better sensor response than a conventional design partly because antibodies are densely and two-dimensionally immobilized onto the surface of the plasma-polymerized film.
| Original language | English |
|---|---|
| Pages (from-to) | 4649-4652 |
| Number of pages | 4 |
| Journal | Analytical Chemistry |
| Volume | 69 |
| Issue number | 22 |
| Publication status | Published - 1997 Nov 15 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing. / Nakamura, Runa; Muguruma, Hitoshi; Ikebukuro, Kazunori; Sasaki, Satoshi; Nagata, Ryohei; Karube, Isao; Pedersen, Henrik.
In: Analytical Chemistry, Vol. 69, No. 22, 15.11.1997, p. 4649-4652.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing
AU - Nakamura, Runa
AU - Muguruma, Hitoshi
AU - Ikebukuro, Kazunori
AU - Sasaki, Satoshi
AU - Nagata, Ryohei
AU - Karube, Isao
AU - Pedersen, Henrik
PY - 1997/11/15
Y1 - 1997/11/15
N2 - We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed on the gold surfaces are extremely thin (∼100 nm), are homogeneous, demonstrate good adhesion, have a flat profile, and incorporate amino groups to introduce a chemical functionality. A sensor chip made with the film has many advantages when compared with conventionally used designs such as carboxylated dextran hydrogels. For example, a sensor chip made with the film shows a better sensor response than a conventional design partly because antibodies are densely and two-dimensionally immobilized onto the surface of the plasma-polymerized film.
AB - We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed on the gold surfaces are extremely thin (∼100 nm), are homogeneous, demonstrate good adhesion, have a flat profile, and incorporate amino groups to introduce a chemical functionality. A sensor chip made with the film has many advantages when compared with conventionally used designs such as carboxylated dextran hydrogels. For example, a sensor chip made with the film shows a better sensor response than a conventional design partly because antibodies are densely and two-dimensionally immobilized onto the surface of the plasma-polymerized film.
UR - http://www.scopus.com/inward/record.url?scp=0031273209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031273209&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0031273209
VL - 69
SP - 4649
EP - 4652
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 22
ER -