A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing

Runa Nakamura, Hitoshi Muguruma, Kazunori Ikebukuro, Satoshi Sasaki, Ryohei Nagata, Isao Karube, Henrik Pedersen

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed on the gold surfaces are extremely thin (∼100 nm), are homogeneous, demonstrate good adhesion, have a flat profile, and incorporate amino groups to introduce a chemical functionality. A sensor chip made with the film has many advantages when compared with conventionally used designs such as carboxylated dextran hydrogels. For example, a sensor chip made with the film shows a better sensor response than a conventional design partly because antibodies are densely and two-dimensionally immobilized onto the surface of the plasma-polymerized film.

Original languageEnglish
Pages (from-to)4649-4652
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number22
Publication statusPublished - 1997 Nov 15
Externally publishedYes

Fingerprint

Surface plasmon resonance
Plasmas
ethylenediamine
Gold
Sensors
Hydrogels
Dextrans
Adhesion
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Nakamura, R., Muguruma, H., Ikebukuro, K., Sasaki, S., Nagata, R., Karube, I., & Pedersen, H. (1997). A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing. Analytical Chemistry, 69(22), 4649-4652.

A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing. / Nakamura, Runa; Muguruma, Hitoshi; Ikebukuro, Kazunori; Sasaki, Satoshi; Nagata, Ryohei; Karube, Isao; Pedersen, Henrik.

In: Analytical Chemistry, Vol. 69, No. 22, 15.11.1997, p. 4649-4652.

Research output: Contribution to journalArticle

Nakamura, R, Muguruma, H, Ikebukuro, K, Sasaki, S, Nagata, R, Karube, I & Pedersen, H 1997, 'A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing', Analytical Chemistry, vol. 69, no. 22, pp. 4649-4652.
Nakamura R, Muguruma H, Ikebukuro K, Sasaki S, Nagata R, Karube I et al. A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing. Analytical Chemistry. 1997 Nov 15;69(22):4649-4652.
Nakamura, Runa ; Muguruma, Hitoshi ; Ikebukuro, Kazunori ; Sasaki, Satoshi ; Nagata, Ryohei ; Karube, Isao ; Pedersen, Henrik. / A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing. In: Analytical Chemistry. 1997 ; Vol. 69, No. 22. pp. 4649-4652.
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