A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel

Tomohisa Ogawa, Nirai Matsunaga, Saori Inomata, Masato Tanaka, Nobuyuki Futai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have developed a cast microfluidic chip that contains a thin (∼2 μm wide) microchannel that is smoothly connected to thick microfluidics. The thin line features having high aspect ratio for a low-cost photolithography in which an emulsion photomask was used (1-1 ∼ 1-3) were fabricated by exposing SU-8 photoresist to diffused 185 nm UV light emitted by a low-cost ozone lamp from the backside of the substrate to ensure sufficient crosslinking of small regions of the SU-8 photoresist. An H-shaped microfluidic configuration was used, in which the thin channel maintained constant diffusion fronts beyond purely static diffusion. We also demonstrated the long-term effects of a gradient of nerve growth factor on axon elongation by primary neurons cultured in the micro channel.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages5521-5524
Number of pages4
DOIs
Publication statusPublished - 2013
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka
Duration: 2013 Jul 32013 Jul 7

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
CityOsaka
Period13/7/313/7/7

Fingerprint

Microchannels
Microfluidics
Aspect ratio
Photoresists
Photomasks
Photolithography
Electric lamps
Ultraviolet radiation
Crosslinking
Ozone
Neurons
Emulsions
Costs
Elongation
Substrates

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Ogawa, T., Matsunaga, N., Inomata, S., Tanaka, M., & Futai, N. (2013). A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 5521-5524). [6610800] https://doi.org/10.1109/EMBC.2013.6610800

A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. / Ogawa, Tomohisa; Matsunaga, Nirai; Inomata, Saori; Tanaka, Masato; Futai, Nobuyuki.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 5521-5524 6610800.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogawa, T, Matsunaga, N, Inomata, S, Tanaka, M & Futai, N 2013, A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6610800, pp. 5521-5524, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, 13/7/3. https://doi.org/10.1109/EMBC.2013.6610800
Ogawa T, Matsunaga N, Inomata S, Tanaka M, Futai N. A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 5521-5524. 6610800 https://doi.org/10.1109/EMBC.2013.6610800
Ogawa, Tomohisa ; Matsunaga, Nirai ; Inomata, Saori ; Tanaka, Masato ; Futai, Nobuyuki. / A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. pp. 5521-5524
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