A single oral administration of theaflavins increases energy expenditure and the expression of metabolic genes

Naoto Kudo, Yasunori Arai, Yoshitomo Suhara, Takeshi Ishii, Tsutomu Nakayama, Naomi Osakabe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Theaflavins are polyphenols found in black tea, whose physiological activities are not well understood. This study on mice evaluated the influence of a single oral administration of theaflavins on energy metabolism by monitoring the initial metabolic changess in skeletal muscle and brown adipose tissue (BAT). Oxygen consumption (VO2) and energy expenditure (EE) were increased significantly in mice treated with theaflavin rich fraction (TF) compared with the group administered vehicle alone. There was no difference in locomotor activity. Fasting mice were euthanized under anesthesia before and 2 and 5, 20-hr after treatment with TF or vehicle. The mRNA levels of uncoupling protein-1 (UCP-1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) in BAT were increased significantly 2-hr after administration of TF. The levels of UCP-3 and PGC-1α in the gastrocnemius muscle were increased significantly 2 and 5-hr after administration of TF. The concentration of phosphorylated AMP-activated protein kinase (AMPK) 1α was also increased significantly in the gastrocnemius 2 and 5-hr after treatment with TF. These results indicate that TF significantly enhances systemic energy expenditure, as evidenced by an increase in expression of metabolic genes.

Original languageEnglish
Article numbere0137809
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - 2015 Sep 16

Fingerprint

theaflavins
energy expenditure
oral administration
Genes
genes
brown adipose tissue
Muscle
mice
Tissue
AMP-activated protein kinase
AMP-Activated Protein Kinases
black tea
Energy Metabolism
theaflavin
Polyphenols
energy metabolism
oxygen consumption
locomotion
fasting
skeletal muscle

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A single oral administration of theaflavins increases energy expenditure and the expression of metabolic genes. / Kudo, Naoto; Arai, Yasunori; Suhara, Yoshitomo; Ishii, Takeshi; Nakayama, Tsutomu; Osakabe, Naomi.

In: PLoS One, Vol. 10, No. 9, e0137809, 16.09.2015.

Research output: Contribution to journalArticle

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