A two-dimensional fluorometric imaging "sniffer camera" of ethanol vapor for evaluation of alcohol metabolism using enzymatic reaction

Takahiro Arakawa, Koji Toma, Kohji Mitsubayashi, Kenta Iitani, Toshiyuki Sato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Various volatile organic compounds can be found in human transpiration, breath and body odor. Therefore, a novel two-dimensional fluorometric imaging system, known as a "sniffer camera" for gaseous ethanol emissions from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multi UV-LED excitation sheet and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of NADH, which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed a rapidly and accurately responses and a visible measurement, which could lead an analysis to metabolism function at real time.

Original languageEnglish
Title of host publication2015 IEEE SENSORS - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479982028
DOIs
Publication statusPublished - 2015 Dec 31
Externally publishedYes
Event14th IEEE SENSORS - Busan, Korea, Republic of
Duration: 2015 Nov 12015 Nov 4

Publication series

Name2015 IEEE SENSORS - Proceedings

Other

Other14th IEEE SENSORS
Country/TerritoryKorea, Republic of
CityBusan
Period15/11/115/11/4

Keywords

  • alcohol dehydrogenase
  • ethanol vapor
  • fluorescence
  • imaging
  • NADH

ASJC Scopus subject areas

  • Instrumentation
  • Electronic, Optical and Magnetic Materials
  • Spectroscopy
  • Electrical and Electronic Engineering

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