Abstract
Background: Rosmarinic acid (RA) is a natural polyphenolic substance contained in many Lamiaceae herbs such as Perilla frutescens. Previous studies have shown RA has antioxidative and anti-inflammatory activity. However, little is known on the absorption, metabolism, degradation and excretion of RA. Aim of the study: The aim of this study in healthy humans was to determine the absorption, metabolism, and urinary excretion of RA after a single intake of perilla extract (PE). Method: Six healthy men (mean age 37.2 ± 6.2 y and mean body mass index 22.0 ± 1.9 kg/m2) were enrolled in the study that was a crossover design involving single intakes of PE containing 200 mg RA and placebo with a 10 day interval between treatments. Blood samples were collected before intake and at designated time intervals, while urine samples were collected over the periods 0-6 h, 6-24 h and 24-48 h after intake. RA and its related metabolites in plasma and urine were measured by LC-MS. Results: RA, methylated RA (methyl-RA), caffeic acid (CAA), ferulic acid (FA) and a trace of m-coumaric acid (COA) were detected in the urine after intake of PE. In plasma, RA, methyl-RA and FA were detected, with maximum levels obtained 0.5, 2 and 0.5 h after intake of PE, respectively. The majority of these components in both plasma and urine were present as conjugated forms (glucuronide and/or sulfated). The proportion of RA and its related metabolites excreted in the urine was 6.3 ± 2.2% of the total dose, with approximately 75% of these components being excreted within 6 h after intake of PE. Conclusions: RA contained in PE was absorbed, conjugated and methylated following intake, with a small proportion of RA being degraded into various components, such as conjugated forms of CAA, FA and COA. These metabolites were then rapidly excreted in the urine.
| Original language | English |
|---|---|
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | European Journal of Nutrition |
| Volume | 44 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2005 Jan |
| Externally published | Yes |
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Keywords
- Absorption
- Human
- Metabolism
- Perilla frutescens
- Rosmarinic acid
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science
Cite this
Absorption, metabolism, degradation and urinary excretion of rosmarinic acid after intake of Perilla frutescens extract in humans. / Baba, Seigo; Osakabe, Naomi; Natsume, Midori; Yasuda, Akiko; Muto, Yuko; Hiyoshi, Kyoko; Takano, Hirohisa; Yoshikawa, Toshikazu; Terao, Junji.
In: European Journal of Nutrition, Vol. 44, No. 1, 01.2005, p. 1-9.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Absorption, metabolism, degradation and urinary excretion of rosmarinic acid after intake of Perilla frutescens extract in humans
AU - Baba, Seigo
AU - Osakabe, Naomi
AU - Natsume, Midori
AU - Yasuda, Akiko
AU - Muto, Yuko
AU - Hiyoshi, Kyoko
AU - Takano, Hirohisa
AU - Yoshikawa, Toshikazu
AU - Terao, Junji
PY - 2005/1
Y1 - 2005/1
N2 - Background: Rosmarinic acid (RA) is a natural polyphenolic substance contained in many Lamiaceae herbs such as Perilla frutescens. Previous studies have shown RA has antioxidative and anti-inflammatory activity. However, little is known on the absorption, metabolism, degradation and excretion of RA. Aim of the study: The aim of this study in healthy humans was to determine the absorption, metabolism, and urinary excretion of RA after a single intake of perilla extract (PE). Method: Six healthy men (mean age 37.2 ± 6.2 y and mean body mass index 22.0 ± 1.9 kg/m2) were enrolled in the study that was a crossover design involving single intakes of PE containing 200 mg RA and placebo with a 10 day interval between treatments. Blood samples were collected before intake and at designated time intervals, while urine samples were collected over the periods 0-6 h, 6-24 h and 24-48 h after intake. RA and its related metabolites in plasma and urine were measured by LC-MS. Results: RA, methylated RA (methyl-RA), caffeic acid (CAA), ferulic acid (FA) and a trace of m-coumaric acid (COA) were detected in the urine after intake of PE. In plasma, RA, methyl-RA and FA were detected, with maximum levels obtained 0.5, 2 and 0.5 h after intake of PE, respectively. The majority of these components in both plasma and urine were present as conjugated forms (glucuronide and/or sulfated). The proportion of RA and its related metabolites excreted in the urine was 6.3 ± 2.2% of the total dose, with approximately 75% of these components being excreted within 6 h after intake of PE. Conclusions: RA contained in PE was absorbed, conjugated and methylated following intake, with a small proportion of RA being degraded into various components, such as conjugated forms of CAA, FA and COA. These metabolites were then rapidly excreted in the urine.
AB - Background: Rosmarinic acid (RA) is a natural polyphenolic substance contained in many Lamiaceae herbs such as Perilla frutescens. Previous studies have shown RA has antioxidative and anti-inflammatory activity. However, little is known on the absorption, metabolism, degradation and excretion of RA. Aim of the study: The aim of this study in healthy humans was to determine the absorption, metabolism, and urinary excretion of RA after a single intake of perilla extract (PE). Method: Six healthy men (mean age 37.2 ± 6.2 y and mean body mass index 22.0 ± 1.9 kg/m2) were enrolled in the study that was a crossover design involving single intakes of PE containing 200 mg RA and placebo with a 10 day interval between treatments. Blood samples were collected before intake and at designated time intervals, while urine samples were collected over the periods 0-6 h, 6-24 h and 24-48 h after intake. RA and its related metabolites in plasma and urine were measured by LC-MS. Results: RA, methylated RA (methyl-RA), caffeic acid (CAA), ferulic acid (FA) and a trace of m-coumaric acid (COA) were detected in the urine after intake of PE. In plasma, RA, methyl-RA and FA were detected, with maximum levels obtained 0.5, 2 and 0.5 h after intake of PE, respectively. The majority of these components in both plasma and urine were present as conjugated forms (glucuronide and/or sulfated). The proportion of RA and its related metabolites excreted in the urine was 6.3 ± 2.2% of the total dose, with approximately 75% of these components being excreted within 6 h after intake of PE. Conclusions: RA contained in PE was absorbed, conjugated and methylated following intake, with a small proportion of RA being degraded into various components, such as conjugated forms of CAA, FA and COA. These metabolites were then rapidly excreted in the urine.
KW - Absorption
KW - Human
KW - Metabolism
KW - Perilla frutescens
KW - Rosmarinic acid
UR - http://www.scopus.com/inward/record.url?scp=13844253586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844253586&partnerID=8YFLogxK
U2 - 10.1007/s00394-004-0482-2
DO - 10.1007/s00394-004-0482-2
M3 - Article
VL - 44
SP - 1
EP - 9
JO - European Journal of Nutrition
JF - European Journal of Nutrition
SN - 1436-6207
IS - 1
ER -