Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems

Dongeun Huh, Hideki Fujioka, Yi Chung Tung, Nobuyuki Futai, Robert Paine, James B. Grotberg, Shuichi Takayama

Research output: Contribution to journalArticle

289 Citations (Scopus)

Abstract

We describe a microfabricated airway system integrated with computerized air-liquid two-phase microfluidics that enables on-chip engineering of human airway epithelia and precise reproduction of physiologic or pathologic liquid plug flows found in the respiratory system. Using this device, we demonstrate cellular-level lung injury under flow conditions that cause symptoms characteristic of a wide range of pulmonary diseases. Specifically, propagation and rupture of liquid plugs that simulate surfactant-deficient reopening of closed airways lead to significant injury of small airway epithelial cells by generating deleterious fluid mechanical stresses. We also show that the explosive pressure waves produced by plug rupture enable detection of the mechanical cellular injury as crackling sounds.

Original languageEnglish
Pages (from-to)18886-18891
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number48
DOIs
Publication statusPublished - 2007 Nov 27

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Keywords

  • Airway reopening
  • Mechanical forces
  • Microfluidic cell culture
  • Small airway epithelial cells

ASJC Scopus subject areas

  • General

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