Acylated anthocyanins derived from purple carrot (: Daucus carota L.) induce elevation of blood flow in rat cremaster arteriole

Ayaka Tsutsumi, Yukari Horikoshi, Taiki Fushimi, Akiko Saito, Ryo Koizumi, Yasuyuki Fujii, Qing Qiang Hu, Yoshihisa Hirota, Koichi Aizawa, Naomi Osakabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Acylated anthocyanins are more stable than monomeric anthocyanins, but little is known about their physiological effects. We evaluated the hemodynamic effects of single intragastric doses of purple carrot (Daucus carota L.) anthocyanin (PCA) and two monomeric anthocyanins, cyanidin 3-O-glycoside (C3G) and delphinidin 3-O-ruthenoside (D3R). PCA, C3G, or D3R was administered orally to rat and blood flow in the cremaster artery was measured for 60 min using a laser Doppler blood flow meter. After measurements, the aorta of the animal was removed and the extent of phosphorylation of aortic epithelial nitric oxide synthase (eNOS) and Akt were determined by western blotting. PCA (10 mg kg -1 ) or C3G (1 mg kg -1 ) significantly increased rat cremaster arteriole blood flow and phosphorylation of eNOS and Akt; D3G (1 mg kg -1 ) only slightly altered cremaster arteriole blood flow and did not affect the phosphorylation of eNOS and Akt in the aorta. These results suggest that hemodynamic alterations depend more on the chemical structure of anthocyanins, particularly the aglycon, than on the glycoside. In addition, increase of blood flow by a single oral dose of PCA was practically reduced with treatment of carvedilol (CR), a non-specific adrenaline blocker. Blood concentrations of cyanidin or its glycoside 15, 30, or 60 min after the administration of 10 mg kg -1 PCA were below the limit of detection. These hemodynamic changes may have been associated with an indirect adrenergic action induced following a single dose of PCA.

Original languageEnglish
Pages (from-to)1726-1735
Number of pages10
JournalFood and Function
Volume10
Issue number3
DOIs
Publication statusPublished - 2019 Mar 1

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Daucus carota
carrots
blood flow
cyanidin
glycosides
anthocyanins
hemodynamics
nitric oxide synthase
rats
phosphorylation
delphinidin
aorta
dosage
flowmeters
epinephrine
chemical structure
arteries
lasers
mouth
detection limit

ASJC Scopus subject areas

  • Food Science

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Acylated anthocyanins derived from purple carrot ( : Daucus carota L.) induce elevation of blood flow in rat cremaster arteriole. / Tsutsumi, Ayaka; Horikoshi, Yukari; Fushimi, Taiki; Saito, Akiko; Koizumi, Ryo; Fujii, Yasuyuki; Hu, Qing Qiang; Hirota, Yoshihisa; Aizawa, Koichi; Osakabe, Naomi.

In: Food and Function, Vol. 10, No. 3, 01.03.2019, p. 1726-1735.

Research output: Contribution to journalArticle

Tsutsumi, Ayaka ; Horikoshi, Yukari ; Fushimi, Taiki ; Saito, Akiko ; Koizumi, Ryo ; Fujii, Yasuyuki ; Hu, Qing Qiang ; Hirota, Yoshihisa ; Aizawa, Koichi ; Osakabe, Naomi. / Acylated anthocyanins derived from purple carrot ( : Daucus carota L.) induce elevation of blood flow in rat cremaster arteriole. In: Food and Function. 2019 ; Vol. 10, No. 3. pp. 1726-1735.
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