TY - JOUR
T1 - Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′)2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance
AU - Kurosawa, Shigeru
AU - Hirokawa, Tomoya
AU - Kashima, Kazuya
AU - Aizawa, Hidenobu
AU - Park, Jong Won
AU - Tozuka, Mitsuhiro
AU - Yoshimi, Yasuo
AU - Hirano, Katsuhiko
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab')2 fragment of IgG antibody (CRP-F(ab′)2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′)2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′)2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
AB - Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab')2 fragment of IgG antibody (CRP-F(ab′)2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′)2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′)2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
KW - Anti-CRP monoclonal IgG antibody
KW - C-reactive protein (CRP)
KW - Plasma polymerization allylamine and acrylic acid
KW - Quartz crystal microbalance (QCM)
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U2 - 10.2494/photopolymer.15.323
DO - 10.2494/photopolymer.15.323
M3 - Article
AN - SCOPUS:0036356074
VL - 15
SP - 323
EP - 329
JO - Journal of Photopolymer Science and Technology
JF - Journal of Photopolymer Science and Technology
SN - 0914-9244
IS - 2
ER -