Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance

Shigeru Kurosawa, Tomoya Hirokawa, Kazuya Kashima, Hidenobu Aizawa, Jong Won Park, Mitsuhiro Tozuka, Yasuo Yoshimi, Katsuhiko Hirano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalJournal of Photopolymer Science and Technology
Volume15
Issue number2
Publication statusPublished - 2002

Fingerprint

Allylamine
Styrene
Monoclonal antibodies
Quartz crystal microbalances
Antibodies
C-Reactive Protein
Acrylics
Monoclonal Antibodies
Proteins
Plasmas
Adsorption
Acids
Gold
Immunoglobulin G
acrylic acid
Plasma polymerization
Molecular orientation
Antigens

Keywords

  • Anti-CRP monoclonal IgG antibody
  • C-reactive protein (CRP)
  • F(ab′) fragment of anti-CRP monoclonal IgG antibody
  • Plasma polymerization allylamine and acrylic acid
  • Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance. / Kurosawa, Shigeru; Hirokawa, Tomoya; Kashima, Kazuya; Aizawa, Hidenobu; Park, Jong Won; Tozuka, Mitsuhiro; Yoshimi, Yasuo; Hirano, Katsuhiko.

In: Journal of Photopolymer Science and Technology, Vol. 15, No. 2, 2002, p. 323-329.

Research output: Contribution to journalArticle

Kurosawa, Shigeru ; Hirokawa, Tomoya ; Kashima, Kazuya ; Aizawa, Hidenobu ; Park, Jong Won ; Tozuka, Mitsuhiro ; Yoshimi, Yasuo ; Hirano, Katsuhiko. / Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance. In: Journal of Photopolymer Science and Technology. 2002 ; Vol. 15, No. 2. pp. 323-329.
@article{e4077aec9b3d4cecb0e7e15449b4467a,
title = "Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance",
abstract = "Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.",
keywords = "Anti-CRP monoclonal IgG antibody, C-reactive protein (CRP), F(ab′) fragment of anti-CRP monoclonal IgG antibody, Plasma polymerization allylamine and acrylic acid, Quartz crystal microbalance (QCM)",
author = "Shigeru Kurosawa and Tomoya Hirokawa and Kazuya Kashima and Hidenobu Aizawa and Park, {Jong Won} and Mitsuhiro Tozuka and Yasuo Yoshimi and Katsuhiko Hirano",
year = "2002",
language = "English",
volume = "15",
pages = "323--329",
journal = "Journal of Photopolymer Science and Technology",
issn = "0914-9244",
publisher = "Tokai University",
number = "2",

}

TY - JOUR

T1 - Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance

AU - Kurosawa, Shigeru

AU - Hirokawa, Tomoya

AU - Kashima, Kazuya

AU - Aizawa, Hidenobu

AU - Park, Jong Won

AU - Tozuka, Mitsuhiro

AU - Yoshimi, Yasuo

AU - Hirano, Katsuhiko

PY - 2002

Y1 - 2002

N2 - Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.

AB - Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.

KW - Anti-CRP monoclonal IgG antibody

KW - C-reactive protein (CRP)

KW - F(ab′) fragment of anti-CRP monoclonal IgG antibody

KW - Plasma polymerization allylamine and acrylic acid

KW - Quartz crystal microbalance (QCM)

UR - http://www.scopus.com/inward/record.url?scp=0036356074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036356074&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0036356074

VL - 15

SP - 323

EP - 329

JO - Journal of Photopolymer Science and Technology

JF - Journal of Photopolymer Science and Technology

SN - 0914-9244

IS - 2

ER -