Abstract
Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
| Original language | English |
|---|---|
| Pages (from-to) | 323-329 |
| Number of pages | 7 |
| Journal | Journal of Photopolymer Science and Technology |
| Volume | 15 |
| Issue number | 2 |
| Publication status | Published - 2002 |
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Keywords
- Anti-CRP monoclonal IgG antibody
- C-reactive protein (CRP)
- F(ab′) fragment of anti-CRP monoclonal IgG antibody
- Plasma polymerization allylamine and acrylic acid
- Quartz crystal microbalance (QCM)
ASJC Scopus subject areas
- Polymers and Plastics
- Materials Chemistry