Abstract
Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
| Original language | English |
|---|---|
| Pages (from-to) | 323-329 |
| Number of pages | 7 |
| Journal | Journal of Photopolymer Science and Technology |
| Volume | 15 |
| Issue number | 2 |
| Publication status | Published - 2002 |
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Keywords
- Anti-CRP monoclonal IgG antibody
- C-reactive protein (CRP)
- F(ab′) fragment of anti-CRP monoclonal IgG antibody
- Plasma polymerization allylamine and acrylic acid
- Quartz crystal microbalance (QCM)
ASJC Scopus subject areas
- Polymers and Plastics
- Materials Chemistry
Cite this
Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance. / Kurosawa, Shigeru; Hirokawa, Tomoya; Kashima, Kazuya; Aizawa, Hidenobu; Park, Jong Won; Tozuka, Mitsuhiro; Yoshimi, Yasuo; Hirano, Katsuhiko.
In: Journal of Photopolymer Science and Technology, Vol. 15, No. 2, 2002, p. 323-329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance
AU - Kurosawa, Shigeru
AU - Hirokawa, Tomoya
AU - Kashima, Kazuya
AU - Aizawa, Hidenobu
AU - Park, Jong Won
AU - Tozuka, Mitsuhiro
AU - Yoshimi, Yasuo
AU - Hirano, Katsuhiko
PY - 2002
Y1 - 2002
N2 - Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
AB - Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
KW - Anti-CRP monoclonal IgG antibody
KW - C-reactive protein (CRP)
KW - F(ab′) fragment of anti-CRP monoclonal IgG antibody
KW - Plasma polymerization allylamine and acrylic acid
KW - Quartz crystal microbalance (QCM)
UR - http://www.scopus.com/inward/record.url?scp=0036356074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036356074&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0036356074
VL - 15
SP - 323
EP - 329
JO - Journal of Photopolymer Science and Technology
JF - Journal of Photopolymer Science and Technology
SN - 0914-9244
IS - 2
ER -