Adsorption of anti-C-reactive protein monoclonal antibody and its F(ab′) 2 fragment on plasma-polymerized styrene, allylamine and acrylic acid coated with quartz crystal microbalance

Shigeru Kurosawa, Tomoya Hirokawa, Kazuya Kashima, Hidenobu Aizawa, Jong Won Park, Mitsuhiro Tozuka, Yasuo Yoshimi, Katsuhiko Hirano

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10 Citations (Scopus)

Abstract

Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab') 2 fragment of IgG antibody (CRP-F(ab′) 2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′) 2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′) 2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalJournal of Photopolymer Science and Technology
Volume15
Issue number2
Publication statusPublished - 2002

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Keywords

  • Anti-CRP monoclonal IgG antibody
  • C-reactive protein (CRP)
  • F(ab′) fragment of anti-CRP monoclonal IgG antibody
  • Plasma polymerization allylamine and acrylic acid
  • Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry

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