Adsorption of antibody protein onto plasma-polymerized film characterized by atomic force microscopy and quartz crystal microbalance

Hitoshi Muguruma, Satoshi Miura, Naoya Murata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay.

Original languageEnglish
Pages (from-to)649-651
Number of pages3
JournalIEICE Transactions on Electronics
VolumeE90-C
Issue number3
DOIs
Publication statusPublished - 2007 Mar

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Keywords

  • Adsorption
  • Antibody
  • Plasma-polymerized film

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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