All-in-one uniified microfluidic chip for automation of embryonic cell manipulation

Shinya Sakuma, Yoko Yamanishi, Fumihito Arai, Tatsuo Arai, Akiyuki Hasegawa, Tamio Tanikawa, Akihiko Ichikawa, Osamu Satoh, Akihiro Nakayama, Hiroshi Aso, Mitsuhiro Goto, Seiya Takahashi, Kazutsugu Matsukawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and they are fused by an electrical field. For the current study, the microfluidic chip was designed to have 3 parts for this process. Then, all-in-one unified microfluidic chip was successfully fabricated. Basic functions of these parts are verified independently.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1883-1885
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Keywords

  • Cloning
  • Non-contact manipulation
  • μ-TAS

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Sakuma, S., Yamanishi, Y., Arai, F., Arai, T., Hasegawa, A., Tanikawa, T., Ichikawa, A., Satoh, O., Nakayama, A., Aso, H., Goto, M., Takahashi, S., & Matsukawa, K. (2009). All-in-one uniified microfluidic chip for automation of embryonic cell manipulation. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1883-1885). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.