Alteration of the systemic and microcirculation by a single oral dose of flavan-3-ols

Kodai Ingawa, Nozomi Aruga, Yusuke Matsumura, Masahiro Shibata, Naomi Osakabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Several systematic reviews have reported that flow mediated dilatation (FMD) was significantly increased in subjects after ingestion of chocolate that contains flavan-3-ols; however, the mechanisms responsible for this effect are not clear. In this study, we evaluated the effects of a single oral dose of flavan-3-ols on the systemic circulation and microcirculation in the cremaster muscle using intravital video microscopy in vivo. The cremaster muscle in rats was spread over a plastic chamber and a gastric tube was placed into the stomach. Blood flow in the cremasteric artery was determined using a laser Doppler flowmeter, while blood pressure and heart rate were measured by the tail-cuff method. Red blood cell velocity in arterioles and blood flow in the artery were significantly increased 5 min after the administration of 10 mg/kg flavan-3-ols compared with distilled water treatment. The number of capillaries recruited in the cremaster muscle was also significantly increased 15 min after treatment. Microscopic observation confirmed that increased shear stress on endothelial cells was maintained during the measurement period. The mean arterial blood pressure and heart rate were also significantly elevated soon after administration and returned to baseline before the end of the observation period. Plasma nitrate and nitrite levels, and NO phosphorylation of aortic tissue were significantly increased at 60 min after administration of flavan-3-ols. According to these results, a single oral dose of flavan-3-ols elevates blood pressure and flow transiently, and these effects induce NO production through increased shear stress on endothelial cells.

Original languageEnglish
Article numbere94853
JournalPLoS One
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 16

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Microcirculation
Blood pressure
flavanols
Muscle
mouth
Blood
Endothelial cells
Shear stress
blood flow
blood pressure
dosage
Phosphorylation
Flowmeters
muscles
arteries
shear stress
endothelial cells
heart rate
Nitrites
Water treatment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Alteration of the systemic and microcirculation by a single oral dose of flavan-3-ols. / Ingawa, Kodai; Aruga, Nozomi; Matsumura, Yusuke; Shibata, Masahiro; Osakabe, Naomi.

In: PLoS One, Vol. 9, No. 4, e94853, 16.04.2014.

Research output: Contribution to journalArticle

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