Altered cellular responses to serum mitogens, including platelet-derived growth factor, in cultured smooth muscle cells derived from arteries of patients with moyamoya disease

Masaru Aoyagi, Naomi Fukai, Hiroshi Sakamoto, Tadashi Shinkai, Yoshiharu Matsushima, Mari Yamamoto, Kiyotaka Yamamoto

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Abstract

Progressive stenosis or occlusion of bilateral internal carotid arteries by fibrocellular intimai thickening results in cerebral ischemia in moyamoya disease. The etiology is unknown. We examined cultured arterial smooth muscle cells (SMC) from scalp arteries of five patients with moyamoya disease. In this study we investigated the responsiveness of the cells in culture to serum mitogens including platelet-derived growth factor (PDGF), a major mitogen of SMC, and compared the response to that of cells derived from age-matched control patients. SMC from patients with moyamoya disease proliferated less rapidly in a medium with 15% serum than did control SMC and responded poorly to the addition of PDGF to 5% serum. PDGF alone did not stimulate SMC in a quiescent state to initiate DNA synthesis in moyamoya disease, without serum factors other than bovine serum albumin, though it significantly stimulated the controls. Simultaneous additions of epidermal growth factor, insulin-like growth factor-I, and PDGF stimulated initiation of DNA synthesis in cells from moyamoya disease, but not as much as PDGF alone did in the controls. Although direct correlations with the pathogenesis of the disease remain to be clarified, the results indicate altered interrelations between serum factors and the cellular responses in vessels of moyamoya disease.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalJournal of Cellular Physiology
Volume147
Issue number2
Publication statusPublished - 1991 May
Externally publishedYes

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Platelet-Derived Growth Factor
Mitogens
Muscle
Cells
DNA
Bovine Serum Albumin
Insulin-Like Growth Factor I
Epidermal Growth Factor

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Altered cellular responses to serum mitogens, including platelet-derived growth factor, in cultured smooth muscle cells derived from arteries of patients with moyamoya disease. / Aoyagi, Masaru; Fukai, Naomi; Sakamoto, Hiroshi; Shinkai, Tadashi; Matsushima, Yoshiharu; Yamamoto, Mari; Yamamoto, Kiyotaka.

In: Journal of Cellular Physiology, Vol. 147, No. 2, 05.1991, p. 191-198.

Research output: Contribution to journalArticle

Aoyagi, Masaru ; Fukai, Naomi ; Sakamoto, Hiroshi ; Shinkai, Tadashi ; Matsushima, Yoshiharu ; Yamamoto, Mari ; Yamamoto, Kiyotaka. / Altered cellular responses to serum mitogens, including platelet-derived growth factor, in cultured smooth muscle cells derived from arteries of patients with moyamoya disease. In: Journal of Cellular Physiology. 1991 ; Vol. 147, No. 2. pp. 191-198.
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