Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4

Kenichi Iwata, Azlin Azlan, Hironori Yamakawa, Toshio Omori

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9 Citations (Scopus)


This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53. mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (~. 1.60. mM) was reached after 8. days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.

Original languageEnglish
Pages (from-to)415-418
Number of pages4
JournalJournal of Bioscience and Bioengineering
Issue number4
Publication statusPublished - 2010 Oct 1



  • Ammonia accumulation
  • Lysobacter
  • Nitrogen-fixing bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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