Abstract
This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53. mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (~. 1.60. mM) was reached after 8. days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
| Original language | English |
|---|---|
| Pages (from-to) | 415-418 |
| Number of pages | 4 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 110 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2010 Oct |
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Keywords
- Ammonia accumulation
- Lysobacter
- Nitrogen-fixing bacteria
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4. / Iwata, Kenichi; Azlan, Azlin; Yamakawa, Hironori; Omori, Toshio.
In: Journal of Bioscience and Bioengineering, Vol. 110, No. 4, 10.2010, p. 415-418.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4
AU - Iwata, Kenichi
AU - Azlan, Azlin
AU - Yamakawa, Hironori
AU - Omori, Toshio
PY - 2010/10
Y1 - 2010/10
N2 - This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53. mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (~. 1.60. mM) was reached after 8. days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
AB - This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53. mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (~. 1.60. mM) was reached after 8. days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
KW - Ammonia accumulation
KW - Lysobacter
KW - Nitrogen-fixing bacteria
UR - http://www.scopus.com/inward/record.url?scp=77956650157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956650157&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2010.05.006
DO - 10.1016/j.jbiosc.2010.05.006
M3 - Article
VL - 110
SP - 415
EP - 418
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 4
ER -