Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4

Kenichi Iwata, Azlin Azlan, Hironori Yamakawa, Toshio Omori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53. mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (~. 1.60. mM) was reached after 8. days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.

Original languageEnglish
Pages (from-to)415-418
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume110
Issue number4
DOIs
Publication statusPublished - 2010 Oct

Fingerprint

Ammonia
Bacteria
Nitrogen
Glucose
Molybdenum
Manganese
Ammonium Compounds
Carbon
Iron
Ions

Keywords

  • Ammonia accumulation
  • Lysobacter
  • Nitrogen-fixing bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4. / Iwata, Kenichi; Azlan, Azlin; Yamakawa, Hironori; Omori, Toshio.

In: Journal of Bioscience and Bioengineering, Vol. 110, No. 4, 10.2010, p. 415-418.

Research output: Contribution to journalArticle

Iwata, Kenichi ; Azlan, Azlin ; Yamakawa, Hironori ; Omori, Toshio. / Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4. In: Journal of Bioscience and Bioengineering. 2010 ; Vol. 110, No. 4. pp. 415-418.
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