Abstract
This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53. mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (~. 1.60. mM) was reached after 8. days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
Original language | English |
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Pages (from-to) | 415-418 |
Number of pages | 4 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 110 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2010 Oct 1 |
Keywords
- Ammonia accumulation
- Lysobacter
- Nitrogen-fixing bacteria
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology