Amperometric biosensor based on carbon nanotube functionalized by redox plasma-polymerized film

Tatsuya Hoshino, Hitoshi Muguruma

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel fabrication approach for the amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a plasma-polymerized film (PPF), and enzyme glucose oxidase (GOD) is reported. The configuration of the electrochemical electrode is sequentially composed of sputtered gold, lower acetonitrile PPF, CNT, redox PPF, GOD, and upper acetonitrile PPF (denoted as PPF/GOD/Redox-PPF/CNT/PPF/Au). The lower acetonitrile PPF deposited on Au acts as a permselective membrane, and as a scaffold for CNT layer formation. The upper acetonirile PPF directly deposited on GOD acts as a matrix for enzyme immobilization. The redox PPF polymerized by a monomer of dimethlyaminomethlyferrocene (DAF) is directly deposited onto CNTs. The surface of the functionalized CNT has redox sites of ferrocene groups that shuttle electrons from CNTs to the sensing surface of the Au electrode. The synergy between the redox PPF and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 2.0μAmM-1 cm -2 at +0.4 V vs Ag/AgCl, a linear dynamic range of 4.9-27mM, and a response time of 5 s.

Original languageEnglish
Article number085202
JournalJapanese Journal of Applied Physics
Volume50
Issue number8 PART 1
DOIs
Publication statusPublished - 2011 Aug

Fingerprint

bioinstrumentation
Biosensors
Carbon nanotubes
carbon nanotubes
Plasmas
Glucose oxidase
glucose
oxidase
Acetonitrile
acetonitrile
Oxidation-Reduction
enzymes
Permselective membranes
Electrochemical electrodes
Enzyme immobilization
Multilayer films
electrodes
sensitivity
Scaffolds
immobilization

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Amperometric biosensor based on carbon nanotube functionalized by redox plasma-polymerized film. / Hoshino, Tatsuya; Muguruma, Hitoshi.

In: Japanese Journal of Applied Physics, Vol. 50, No. 8 PART 1, 085202, 08.2011.

Research output: Contribution to journalArticle

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