Amperometric biosensor based on glucose dehydrogenase and plasma-polymerized thin films

Atsunori Hiratsuka, Kohta Fujisawa, Hitoshi Muguruma

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A novel design is described for an amperometric biosensor based on NAD(P)-dependent glucose dehydrogenase (GDH) combined with a plasma-polymerized thin film (PPF). The GDH is sandwiched between several nanometer thick acetonitrile PPFs on a sputtered gold electrode (PPF/GDH/PPF/Au). The lower PPF layer plays the role as an interface between enzyme and electrode because it is extremely thin, adheres well to the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. The upper PPF layer (overcoating) was directly deposited on immobilized GDH. The optimized amperometric biosensor characteristics covered 2.5 - 26 mM glucose concentration at +0.6 V of applied potential; the least-squares slope was 320 nA mM -1 cm -2 and the correlation coefficient was 0.990. Unlike conventional wet-chemical processes that are incompatible with mass production techniques, this dry-chemistry procedure has great potential for enabling high-throughput production of bioelectronic devices. 2008

Original languageEnglish
Pages (from-to)483-486
Number of pages4
JournalAnalytical Sciences
Volume24
Issue number4
DOIs
Publication statusPublished - 2008 Apr

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Amperometric biosensor based on glucose dehydrogenase and plasma-polymerized thin films'. Together they form a unique fingerprint.

  • Cite this