Amperometric biosensor based on glucose dehydrogenase and plasma-polymerized thin films

Atsunori Hiratsuka, Kohta Fujisawa, Hitoshi Muguruma

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel design is described for an amperometric biosensor based on NAD(P)-dependent glucose dehydrogenase (GDH) combined with a plasma-polymerized thin film (PPF). The GDH is sandwiched between several nanometer thick acetonitrile PPFs on a sputtered gold electrode (PPF/GDH/PPF/Au). The lower PPF layer plays the role as an interface between enzyme and electrode because it is extremely thin, adheres well to the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. The upper PPF layer (overcoating) was directly deposited on immobilized GDH. The optimized amperometric biosensor characteristics covered 2.5 - 26 mM glucose concentration at +0.6 V of applied potential; the least-squares slope was 320 nA mM-1 cm-2 and the correlation coefficient was 0.990. Unlike conventional wet-chemical processes that are incompatible with mass production techniques, this dry-chemistry procedure has great potential for enabling high-throughput production of bioelectronic devices. 2008

Original languageEnglish
Pages (from-to)483-486
Number of pages4
JournalAnalytical Sciences
Volume24
Issue number4
DOIs
Publication statusPublished - 2008 Apr

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Glucose 1-Dehydrogenase
Biosensors
Plasmas
Thin films
Electrodes
Gold
NAD
Throughput
Glucose
Substrates
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Amperometric biosensor based on glucose dehydrogenase and plasma-polymerized thin films. / Hiratsuka, Atsunori; Fujisawa, Kohta; Muguruma, Hitoshi.

In: Analytical Sciences, Vol. 24, No. 4, 04.2008, p. 483-486.

Research output: Contribution to journalArticle

Hiratsuka, Atsunori ; Fujisawa, Kohta ; Muguruma, Hitoshi. / Amperometric biosensor based on glucose dehydrogenase and plasma-polymerized thin films. In: Analytical Sciences. 2008 ; Vol. 24, No. 4. pp. 483-486.
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