Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes

Shin Ichiro Imabayashi; Young Tae Kong; Masayoshi Watanabe

doi:10.1246/cl.2000.1020

Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes

Shin Ichiro Imabayashi, Young Tae Kong, Masayoshi Watanabe

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 μmol dm^-3 with a linear relationship up to 25 μmol dm^-3. The reduction current of oxidized polyphenol intermediate formed during the enzymatic reaction in the presence of H₂O₂ is proportional to the concentration of polyphenol. The present method is applicable to the determination of the total polyphenol content in wine and tea.

Original language	English
Pages (from-to)	1020-1021
Number of pages	2
Journal	Chemistry Letters
Issue number	9
DOIs	https://doi.org/10.1246/cl.2000.1020
Publication status	Published - 2000
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.2000.1020

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title = "Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes",

abstract = "Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 μmol dm-3 with a linear relationship up to 25 μmol dm-3. The reduction current of oxidized polyphenol intermediate formed during the enzymatic reaction in the presence of H2O2 is proportional to the concentration of polyphenol. The present method is applicable to the determination of the total polyphenol content in wine and tea.",

author = "Imabayashi, {Shin Ichiro} and Kong, {Young Tae} and Masayoshi Watanabe",

year = "2000",

doi = "10.1246/cl.2000.1020",

language = "English",

pages = "1020--1021",

journal = "Chemistry Letters",

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T1 - Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes

AU - Imabayashi, Shin Ichiro

AU - Kong, Young Tae

AU - Watanabe, Masayoshi

PY - 2000

Y1 - 2000

N2 - Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 μmol dm-3 with a linear relationship up to 25 μmol dm-3. The reduction current of oxidized polyphenol intermediate formed during the enzymatic reaction in the presence of H2O2 is proportional to the concentration of polyphenol. The present method is applicable to the determination of the total polyphenol content in wine and tea.

AB - Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 μmol dm-3 with a linear relationship up to 25 μmol dm-3. The reduction current of oxidized polyphenol intermediate formed during the enzymatic reaction in the presence of H2O2 is proportional to the concentration of polyphenol. The present method is applicable to the determination of the total polyphenol content in wine and tea.

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U2 - 10.1246/cl.2000.1020

DO - 10.1246/cl.2000.1020

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SP - 1020

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JO - Chemistry Letters

JF - Chemistry Letters

IS - 9

ER -

Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes

Abstract

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