Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes

Shin Ichiro Imabayashi, Young Tae Kong, Masayoshi Watanabe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 μmol dm-3 with a linear relationship up to 25 μmol dm-3. The reduction current of oxidized polyphenol intermediate formed during the enzymatic reaction in the presence of H2O2 is proportional to the concentration of polyphenol. The present method is applicable to the determination of the total polyphenol content in wine and tea.

Original languageEnglish
Pages (from-to)1020-1021
Number of pages2
JournalChemistry Letters
Issue number9
DOIs
Publication statusPublished - 2000 Jan 1

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes'. Together they form a unique fingerprint.

  • Cite this