Analysis of peripheral lymphocyte subsets and lymphocytophilic antibodies in ulcerative colitis defined by monoclonal antibody

Mamoru Watanabe, Sadakazu Aiso, Toshifumi Hibi, Takeshi Yoshida, Kensuke Kobayashi, Hitoshi Asakura, Masaharu Tsuchiya, Sumiaki Tsuru

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T cell subsets of peripheral blood lymphocytes were assessed in 27 patients with ulcerative colitis by a fluorescence activated cell sorter, using monoclonal antibodies to the surface antigens of suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cells. The population of suppressor T cells was decreased in the the lymphocyte from patients with active ulcerative colitis (8.2±3.6%,p<0.01), compared with those from normal controls (24.6±3.6%). Furthermore, the binding capacity of each lymphocytes from patients with ulcerative colitis to Anti-Leu-2a was decreased. The relationship between lymphocytophilic antibodies found in patients with ulcerative colitis and T cell subsets was examined by cell sorting on a fluorescence activated cell sorter. The lymphocytophilic antibodies were demonstrated to be mainly reactive with suppressor T cells, but also reactive with non-suppressor, non-helper T cell subsets and some populations of helper T cells. These results suggest that some other factors except lymphocytophilic antibodies may influence the decrease in suppressor T cells in patients with ulcerative colitis.

Original languageEnglish
Pages (from-to)1418-1423
Number of pages6
JournalNippon Shokakibyo Gakkai Zasshi
Issue number7
Publication statusPublished - 1982 Jan 1


ASJC Scopus subject areas

  • Gastroenterology

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