Analysis of peripheral lymphocyte subsets and lymphocytophilic antibodies in ulcerative colitis defined by monoclonal antibody

Mamoru Watanabe, Sadakazu Aiso, Toshifumi Hibi, Takeshi Yoshida, Kensuke Kobayashi, Hitoshi Asakura, Masaharu Tsuchiya, Sumiaki Tsuru

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

T cell subsets of peripheral blood lymphocytes were assessed in 27 patients with ulcerative colitis by a fluorescence activated cell sorter, using monoclonal antibodies to the surface antigens of suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cells. The population of suppressor T cells was decreased in the the lymphocyte from patients with active ulcerative colitis (8.2±3.6%,p<0.01), compared with those from normal controls (24.6±3.6%). Furthermore, the binding capacity of each lymphocytes from patients with ulcerative colitis to Anti-Leu-2a was decreased. The relationship between lymphocytophilic antibodies found in patients with ulcerative colitis and T cell subsets was examined by cell sorting on a fluorescence activated cell sorter. The lymphocytophilic antibodies were demonstrated to be mainly reactive with suppressor T cells, but also reactive with non-suppressor, non-helper T cell subsets and some populations of helper T cells. These results suggest that some other factors except lymphocytophilic antibodies may influence the decrease in suppressor T cells in patients with ulcerative colitis.

Original languageEnglish
Pages (from-to)1418-1423
Number of pages6
JournalNippon Shokakibyo Gakkai Zasshi
Volume79
Issue number7
DOIs
Publication statusPublished - 1982 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Analysis of peripheral lymphocyte subsets and lymphocytophilic antibodies in ulcerative colitis defined by monoclonal antibody. / Watanabe, Mamoru; Aiso, Sadakazu; Hibi, Toshifumi; Yoshida, Takeshi; Kobayashi, Kensuke; Asakura, Hitoshi; Tsuchiya, Masaharu; Tsuru, Sumiaki.

In: Nippon Shokakibyo Gakkai Zasshi, Vol. 79, No. 7, 01.01.1982, p. 1418-1423.

Research output: Contribution to journalArticle

Watanabe, Mamoru ; Aiso, Sadakazu ; Hibi, Toshifumi ; Yoshida, Takeshi ; Kobayashi, Kensuke ; Asakura, Hitoshi ; Tsuchiya, Masaharu ; Tsuru, Sumiaki. / Analysis of peripheral lymphocyte subsets and lymphocytophilic antibodies in ulcerative colitis defined by monoclonal antibody. In: Nippon Shokakibyo Gakkai Zasshi. 1982 ; Vol. 79, No. 7. pp. 1418-1423.
@article{399350e9d63848abab727a49531cb129,
title = "Analysis of peripheral lymphocyte subsets and lymphocytophilic antibodies in ulcerative colitis defined by monoclonal antibody",
abstract = "T cell subsets of peripheral blood lymphocytes were assessed in 27 patients with ulcerative colitis by a fluorescence activated cell sorter, using monoclonal antibodies to the surface antigens of suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cells. The population of suppressor T cells was decreased in the the lymphocyte from patients with active ulcerative colitis (8.2±3.6{\%},p<0.01), compared with those from normal controls (24.6±3.6{\%}). Furthermore, the binding capacity of each lymphocytes from patients with ulcerative colitis to Anti-Leu-2a was decreased. The relationship between lymphocytophilic antibodies found in patients with ulcerative colitis and T cell subsets was examined by cell sorting on a fluorescence activated cell sorter. The lymphocytophilic antibodies were demonstrated to be mainly reactive with suppressor T cells, but also reactive with non-suppressor, non-helper T cell subsets and some populations of helper T cells. These results suggest that some other factors except lymphocytophilic antibodies may influence the decrease in suppressor T cells in patients with ulcerative colitis.",
author = "Mamoru Watanabe and Sadakazu Aiso and Toshifumi Hibi and Takeshi Yoshida and Kensuke Kobayashi and Hitoshi Asakura and Masaharu Tsuchiya and Sumiaki Tsuru",
year = "1982",
month = "1",
day = "1",
doi = "10.11405/nisshoshi1964.79.1418",
language = "English",
volume = "79",
pages = "1418--1423",
journal = "Japanese Journal of Gastroenterology",
issn = "0446-6586",
publisher = "Japanese Society of Gastroenterology",
number = "7",

}

TY - JOUR

T1 - Analysis of peripheral lymphocyte subsets and lymphocytophilic antibodies in ulcerative colitis defined by monoclonal antibody

AU - Watanabe, Mamoru

AU - Aiso, Sadakazu

AU - Hibi, Toshifumi

AU - Yoshida, Takeshi

AU - Kobayashi, Kensuke

AU - Asakura, Hitoshi

AU - Tsuchiya, Masaharu

AU - Tsuru, Sumiaki

PY - 1982/1/1

Y1 - 1982/1/1

N2 - T cell subsets of peripheral blood lymphocytes were assessed in 27 patients with ulcerative colitis by a fluorescence activated cell sorter, using monoclonal antibodies to the surface antigens of suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cells. The population of suppressor T cells was decreased in the the lymphocyte from patients with active ulcerative colitis (8.2±3.6%,p<0.01), compared with those from normal controls (24.6±3.6%). Furthermore, the binding capacity of each lymphocytes from patients with ulcerative colitis to Anti-Leu-2a was decreased. The relationship between lymphocytophilic antibodies found in patients with ulcerative colitis and T cell subsets was examined by cell sorting on a fluorescence activated cell sorter. The lymphocytophilic antibodies were demonstrated to be mainly reactive with suppressor T cells, but also reactive with non-suppressor, non-helper T cell subsets and some populations of helper T cells. These results suggest that some other factors except lymphocytophilic antibodies may influence the decrease in suppressor T cells in patients with ulcerative colitis.

AB - T cell subsets of peripheral blood lymphocytes were assessed in 27 patients with ulcerative colitis by a fluorescence activated cell sorter, using monoclonal antibodies to the surface antigens of suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cells. The population of suppressor T cells was decreased in the the lymphocyte from patients with active ulcerative colitis (8.2±3.6%,p<0.01), compared with those from normal controls (24.6±3.6%). Furthermore, the binding capacity of each lymphocytes from patients with ulcerative colitis to Anti-Leu-2a was decreased. The relationship between lymphocytophilic antibodies found in patients with ulcerative colitis and T cell subsets was examined by cell sorting on a fluorescence activated cell sorter. The lymphocytophilic antibodies were demonstrated to be mainly reactive with suppressor T cells, but also reactive with non-suppressor, non-helper T cell subsets and some populations of helper T cells. These results suggest that some other factors except lymphocytophilic antibodies may influence the decrease in suppressor T cells in patients with ulcerative colitis.

UR - http://www.scopus.com/inward/record.url?scp=0020155838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020155838&partnerID=8YFLogxK

U2 - 10.11405/nisshoshi1964.79.1418

DO - 10.11405/nisshoshi1964.79.1418

M3 - Article

C2 - 6982986

AN - SCOPUS:0020155838

VL - 79

SP - 1418

EP - 1423

JO - Japanese Journal of Gastroenterology

JF - Japanese Journal of Gastroenterology

SN - 0446-6586

IS - 7

ER -