Abstract
This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.
Original language | English |
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Article number | 1703460 |
Pages (from-to) | 1052-1056 |
Number of pages | 5 |
Journal | IEEE Sensors Journal |
Volume | 6 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2006 Oct 1 |
Keywords
- Anti-C-reactive protein (CRP) antibody
- CRP detection
- Functional monolayer
- Immunosensor
- Quartz crystal microbalance (QCM)
ASJC Scopus subject areas
- Instrumentation
- Electrical and Electronic Engineering