Antibody immobilization on functional monolayers using a quartz crystal microbalance

Hidenobu Aizawa; Yasutoshi Gokita; Jong Won Park; Yasuo Yoshimi; Shigeru Kurosawa

doi:10.1109/JSEN.2006.881368

Antibody immobilization on functional monolayers using a quartz crystal microbalance

Hidenobu Aizawa, Yasutoshi Gokita, Jong Won Park, Yasuo Yoshimi, Shigeru Kurosawa

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation a_max, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm², respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.

Original language	English
Article number	1703460
Pages (from-to)	1052-1056
Number of pages	5
Journal	IEEE Sensors Journal
Volume	6
Issue number	5
DOIs	https://doi.org/10.1109/JSEN.2006.881368
Publication status	Published - 2006 Oct 1

Keywords

Anti-C-reactive protein (CRP) antibody
CRP detection
Functional monolayer
Immunosensor
Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

Instrumentation
Electrical and Electronic Engineering

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title = "Antibody immobilization on functional monolayers using a quartz crystal microbalance",

abstract = "This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.",

keywords = "Anti-C-reactive protein (CRP) antibody, CRP detection, Functional monolayer, Immunosensor, Quartz crystal microbalance (QCM)",

author = "Hidenobu Aizawa and Yasutoshi Gokita and Park, {Jong Won} and Yasuo Yoshimi and Shigeru Kurosawa",

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AU - Aizawa, Hidenobu

AU - Gokita, Yasutoshi

AU - Park, Jong Won

AU - Yoshimi, Yasuo

AU - Kurosawa, Shigeru

PY - 2006/10/1

Y1 - 2006/10/1

N2 - This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.

AB - This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.

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