Abstract
This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.
| Original language | English |
|---|---|
| Article number | 1703460 |
| Pages (from-to) | 1052-1056 |
| Number of pages | 5 |
| Journal | IEEE Sensors Journal |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 Oct |
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Keywords
- Anti-C-reactive protein (CRP) antibody
- CRP detection
- Functional monolayer
- Immunosensor
- Quartz crystal microbalance (QCM)
ASJC Scopus subject areas
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
Antibody immobilization on functional monolayers using a quartz crystal microbalance. / Aizawa, Hidenobu; Gokita, Yasutoshi; Park, Jong Won; Yoshimi, Yasuo; Kurosawa, Shigeru.
In: IEEE Sensors Journal, Vol. 6, No. 5, 1703460, 10.2006, p. 1052-1056.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibody immobilization on functional monolayers using a quartz crystal microbalance
AU - Aizawa, Hidenobu
AU - Gokita, Yasutoshi
AU - Park, Jong Won
AU - Yoshimi, Yasuo
AU - Kurosawa, Shigeru
PY - 2006/10
Y1 - 2006/10
N2 - This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.
AB - This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.
KW - Anti-C-reactive protein (CRP) antibody
KW - CRP detection
KW - Functional monolayer
KW - Immunosensor
KW - Quartz crystal microbalance (QCM)
UR - http://www.scopus.com/inward/record.url?scp=33749524549&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749524549&partnerID=8YFLogxK
U2 - 10.1109/JSEN.2006.881368
DO - 10.1109/JSEN.2006.881368
M3 - Article
AN - SCOPUS:33749524549
VL - 6
SP - 1052
EP - 1056
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
SN - 1530-437X
IS - 5
M1 - 1703460
ER -