TY - JOUR
T1 - Antibody immobilization on functional monolayers using a quartz crystal microbalance
AU - Aizawa, Hidenobu
AU - Gokita, Yasutoshi
AU - Park, Jong Won
AU - Yoshimi, Yasuo
AU - Kurosawa, Shigeru
N1 - Funding Information:
Manuscript received August 5, 2005; revised October 6, 2005. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (16040217) from MEXT for “Application of permselective and biocompatible membranes for the improvement quartz crystal microbalance biosensors” from the Daiwa Anglo-Japanese Foundation and for “Study on monitoring of environmental risk compounds such as dioxins and endocrine disruptors using sensing systems” from the Ministry of the Environment, Japan. The associate editor coordinating the review of this paper and approving it for publication was Dr. Jenny Gun.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2006/10
Y1 - 2006/10
N2 - This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.
AB - This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.
KW - Anti-C-reactive protein (CRP) antibody
KW - CRP detection
KW - Functional monolayer
KW - Immunosensor
KW - Quartz crystal microbalance (QCM)
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U2 - 10.1109/JSEN.2006.881368
DO - 10.1109/JSEN.2006.881368
M3 - Article
AN - SCOPUS:33749524549
VL - 6
SP - 1052
EP - 1056
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
SN - 1530-437X
IS - 5
M1 - 1703460
ER -