Antibody immobilization on functional monolayers using a quartz crystal microbalance

Hidenobu Aizawa, Yasutoshi Gokita, Jong Won Park, Yasuo Yoshimi, Shigeru Kurosawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.

Original languageEnglish
Article number1703460
Pages (from-to)1052-1056
Number of pages5
JournalIEEE Sensors Journal
Volume6
Issue number5
DOIs
Publication statusPublished - 2006 Oct

Fingerprint

Quartz crystal microbalances
quartz crystals
antibodies
immobilization
Antibodies
microbalances
Monolayers
proteins
Proteins
acids
Acids
Monoclonal antibodies
antigens
Antigens
Isotherms
isotherms
Gold
Calibration
gold
curves

Keywords

  • Anti-C-reactive protein (CRP) antibody
  • CRP detection
  • Functional monolayer
  • Immunosensor
  • Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

  • Engineering(all)
  • Electrical and Electronic Engineering

Cite this

Antibody immobilization on functional monolayers using a quartz crystal microbalance. / Aizawa, Hidenobu; Gokita, Yasutoshi; Park, Jong Won; Yoshimi, Yasuo; Kurosawa, Shigeru.

In: IEEE Sensors Journal, Vol. 6, No. 5, 1703460, 10.2006, p. 1052-1056.

Research output: Contribution to journalArticle

Aizawa, Hidenobu ; Gokita, Yasutoshi ; Park, Jong Won ; Yoshimi, Yasuo ; Kurosawa, Shigeru. / Antibody immobilization on functional monolayers using a quartz crystal microbalance. In: IEEE Sensors Journal. 2006 ; Vol. 6, No. 5. pp. 1052-1056.
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