Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein

Haruka Sudo, Hong Jiang, Takashi Yasukawa, Yuichi Hashimoto, Takako Niikura, Masaoki Kawasumi, Shuji Matsuda, Yuji Takeuchi, Sadakazu Aiso, Masaaki Matsuoka, Yoshitake Murayama, Ikuo Nishimoto

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.

Original languageEnglish
Pages (from-to)708-723
Number of pages16
JournalMolecular and Cellular Neuroscience
Volume16
Issue number6
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Sudo, H., Jiang, H., Yasukawa, T., Hashimoto, Y., Niikura, T., Kawasumi, M., ... Nishimoto, I. (2000). Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein. Molecular and Cellular Neuroscience, 16(6), 708-723. https://doi.org/10.1006/mcne.2000.0910

Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein. / Sudo, Haruka; Jiang, Hong; Yasukawa, Takashi; Hashimoto, Yuichi; Niikura, Takako; Kawasumi, Masaoki; Matsuda, Shuji; Takeuchi, Yuji; Aiso, Sadakazu; Matsuoka, Masaaki; Murayama, Yoshitake; Nishimoto, Ikuo.

In: Molecular and Cellular Neuroscience, Vol. 16, No. 6, 01.01.2000, p. 708-723.

Research output: Contribution to journalArticle

Sudo, H, Jiang, H, Yasukawa, T, Hashimoto, Y, Niikura, T, Kawasumi, M, Matsuda, S, Takeuchi, Y, Aiso, S, Matsuoka, M, Murayama, Y & Nishimoto, I 2000, 'Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein', Molecular and Cellular Neuroscience, vol. 16, no. 6, pp. 708-723. https://doi.org/10.1006/mcne.2000.0910
Sudo, Haruka ; Jiang, Hong ; Yasukawa, Takashi ; Hashimoto, Yuichi ; Niikura, Takako ; Kawasumi, Masaoki ; Matsuda, Shuji ; Takeuchi, Yuji ; Aiso, Sadakazu ; Matsuoka, Masaaki ; Murayama, Yoshitake ; Nishimoto, Ikuo. / Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein. In: Molecular and Cellular Neuroscience. 2000 ; Vol. 16, No. 6. pp. 708-723.
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