Abstract
APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.
| Original language | English |
|---|---|
| Pages (from-to) | 708-723 |
| Number of pages | 16 |
| Journal | Molecular and Cellular Neuroscience |
| Volume | 16 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2000 Jan 1 |
| Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
- Cell Biology
Cite this
Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein. / Sudo, Haruka; Jiang, Hong; Yasukawa, Takashi; Hashimoto, Yuichi; Niikura, Takako; Kawasumi, Masaoki; Matsuda, Shuji; Takeuchi, Yuji; Aiso, Sadakazu; Matsuoka, Masaaki; Murayama, Yoshitake; Nishimoto, Ikuo.
In: Molecular and Cellular Neuroscience, Vol. 16, No. 6, 01.01.2000, p. 708-723.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein
AU - Sudo, Haruka
AU - Jiang, Hong
AU - Yasukawa, Takashi
AU - Hashimoto, Yuichi
AU - Niikura, Takako
AU - Kawasumi, Masaoki
AU - Matsuda, Shuji
AU - Takeuchi, Yuji
AU - Aiso, Sadakazu
AU - Matsuoka, Masaaki
AU - Murayama, Yoshitake
AU - Nishimoto, Ikuo
PY - 2000/1/1
Y1 - 2000/1/1
N2 - APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.
AB - APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.
UR - http://www.scopus.com/inward/record.url?scp=0034525215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034525215&partnerID=8YFLogxK
U2 - 10.1006/mcne.2000.0910
DO - 10.1006/mcne.2000.0910
M3 - Article
C2 - 11124892
AN - SCOPUS:0034525215
VL - 16
SP - 708
EP - 723
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
IS - 6
ER -