TY - JOUR
T1 - Antibody-regulated neurotoxic function of cell-surface β-amyloid precursor protein
AU - Sudo, Haruka
AU - Jiang, Hong
AU - Yasukawa, Takashi
AU - Hashimoto, Yuichi
AU - Niikura, Takako
AU - Kawasumi, Masaoki
AU - Matsuda, Shuji
AU - Takeuchi, Yuji
AU - Aiso, Sadakazu
AU - Matsuoka, Masaaki
AU - Murayama, Yoshitake
AU - Nishimoto, Ikuo
PY - 2000
Y1 - 2000
N2 - APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.
AB - APP is a transmembrane precursor of β-amyloid, and its mutations cause early-onset familial Alzheimer's disease. We report a toxic function of normal wild-type APP (wtAPP). Treatment of neuronal F11 cells, immortalized embryonic day 13 neurons, overexpressing wtAPP with anti-APP antibodies caused death. Death was not induced by antibody in parental F11 cells, Death by antibody occurred through cell-surface APP, not through secreted APP, in a pertussis toxin-sensitive manner and was typical apoptosis, not observed in primary astrocytes or glioma cells overexpressing wtAPP, but observed in primary cortical neurons. Cell-surface APP thus performs a toxic function as an extracellularly controllable regulator of neuronal death. This study provides a novel insight into the normal and pathological functions of cell-surface wtAPP.
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U2 - 10.1006/mcne.2000.0910
DO - 10.1006/mcne.2000.0910
M3 - Article
C2 - 11124892
AN - SCOPUS:0034525215
VL - 16
SP - 708
EP - 723
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
IS - 6
ER -