Antioxidative Polyphenols Isolated from Theobroma cacao

Chiaki Sanbongi, Naomi Osakabe, Midori Natsume, Toshio Takizawa, Shuichi Gomi, Toshihiko Osawa

Research output: Contribution to journalArticlepeer-review

207 Citations (Scopus)


The antioxidant components of cacao liquor, which is a major ingredient of chocolate, were isolated with column chromatography and high-performance liquid chromatography. Quercetin and its glucoside were identified by spectrometric methods. Clovamide and deoxyclovamide were characterized by 1H and 13C NMR and MS spectrometry. Their antioxidative activity was measured by peroxide value of linoleic acid and thiobarbituric acid reactive-substance value of erythrocyte ghost membranes and microsomes. In the bulk oil system, clovamide had the strongest antioxidative activity but was less active in the other experiments. In the case of the two hydrophilic systems, flavans such as quercetin and epicatechin were more potently effective than the glucosides. It is considered that chocolate is stable against oxidative deterioration due to the presence of these polyphenolic compounds.

Original languageEnglish
Pages (from-to)454-457
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Issue number2
Publication statusPublished - 1998 Feb
Externally publishedYes


  • Antioxidant
  • Cacao liquor
  • Catechin
  • Clovamide
  • Polyphenols

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


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