APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus

Eric Glasgow, Seung Lim Ryu, Mitsuo Yamashita, Bing Jun Zhang, Noriko Mutsuga, Harold Gainer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Vasopressin (VP) and oxytocin (OT) play critical roles in the regulation of salt and water balance, lactation, and various behaviors and are expressed at very high levels in specific magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS). In addition to the cell-specific expression of the VP and OT genes in these cells, there are other transcripts that are preferentially expressed in the VP or OT MCNs. One such gene, paternally expressed gene 3 (Peg3), is an imprinted gene expressed exclusively from the paternal allele that encodes a Kruppel-type zinc finger-containing protein involved in maternal behavior and is abundantly expressed in the VP-MCNs. We report here the robust expression in the VP-MCNs of an RNA, which we designate APeg3 that is transcribed in the antisense direction to the 3′ untranslated region of the Peg3 gene. The APeg3 mRNA is about 1 kb in size, and the full-length sequence of APeg3, as determined by 5′ and 3′ RACE, contains an open reading frame that predicts a protein of 93 amino acids and is predominantly expressed in VP-MCNs. Both Peg3 and APeg3 gene expression in the VP-MCNs increase during systemic hyperosmolality in vivo, demonstrating that both of these genes are osmoregulated.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalMolecular Brain Research
Volume137
Issue number1-2
DOIs
Publication statusPublished - 2005 Jun 13
Externally publishedYes

Keywords

  • Antisense
  • Hypothalamus
  • Imprinted genes
  • Non-coding RNA
  • Peg 3
  • Supraoptic nucleus
  • Vasopressin

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus. / Glasgow, Eric; Ryu, Seung Lim; Yamashita, Mitsuo; Zhang, Bing Jun; Mutsuga, Noriko; Gainer, Harold.

In: Molecular Brain Research, Vol. 137, No. 1-2, 13.06.2005, p. 143-151.

Research output: Contribution to journalArticle

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