Application of ultrasound for tissue fixation: Combined use with microwave to enhance the effect of chemical fixation

Yasuda Kenjiro, Yamashita Shuji, Shiozawa Masahide, Sadakazu Aiso, Yasui Yoshiaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microwave (MW) has the advantage of accelerating infiltration of fixatives into tissue blocks, but the disadvantage of causing the temperature of the fixatives and tissue blocks to rise. We tried to minimize the temperature elevation during MW irradiation by applying ultrasound (US) in combination with MW oven furnishing US generator. US was uneffective in reducing the rise of temperature, however, it was able to enhance the rapid tissue fixation. Electron microscopic examination showed that the fine structure of organs, such as liver, pancreas, submandibular gland, small intestine, and kidney, was well preserved. Characteristically, the matrix of mitochondria appeared electron-dense, and the marginal zone of zymogen granules in the pancreas was more electron-opaque than the core of the granule. The immunoreactivity of zymogen granules to anti-amylase antibody was strong. The deformation or disorder of cell structure due to the thermal effect and cavitation that can be caused by US was not seen. US can be useful for histologic and immunohistochemical study.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalActa Histochemica et Cytochemica
Volume25
Issue number5
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

Fingerprint

Ultrasonics
Microwaves
Tissue
Fixatives
Enzyme Precursors
Electrons
Acoustic generators
Microwave ovens
Mitochondria
Microwave irradiation
Amylases
Infiltration
Cavitation
Liver
Thermal effects
Temperature
Microscopic examination
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

Cite this

Application of ultrasound for tissue fixation : Combined use with microwave to enhance the effect of chemical fixation. / Kenjiro, Yasuda; Shuji, Yamashita; Masahide, Shiozawa; Aiso, Sadakazu; Yoshiaki, Yasui.

In: Acta Histochemica et Cytochemica, Vol. 25, No. 5, 01.01.1992, p. 237-244.

Research output: Contribution to journalArticle

Kenjiro, Yasuda ; Shuji, Yamashita ; Masahide, Shiozawa ; Aiso, Sadakazu ; Yoshiaki, Yasui. / Application of ultrasound for tissue fixation : Combined use with microwave to enhance the effect of chemical fixation. In: Acta Histochemica et Cytochemica. 1992 ; Vol. 25, No. 5. pp. 237-244.
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