Bacterium-based heavy metal biosorbents: Enhanced uptake of cadmium by E. coli Expressing a metallothionein fused to β-galactosidase

Naoto Yoshida, T. Kato, T. Yoshida, K. Ogawa, Mitsuo Yamashita, Y. Murooka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We investigated the potential utility of a recombinant E. coli that expresses the human metallothionein II gene as a fusion protein with β-galactosidase as a heavy metal biosorbent. E. coli cells expressing the metallothionein fusion demonstrated enhanced binding of Cd2+ compared to cells that lack the metallothionein. It was shown that the metallothionein fusion was capable of efficiently removing Cd2+ from solutions. Approximately 40% of the Cd2+ accumulated by the recombinant cells free in suspension was associated with the outer cell membrane, and 60% of that was present in the cytoplasm.

Original languageEnglish
Pages (from-to)551-558
Number of pages8
JournalBioTechniques
Volume32
Issue number3
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Galactosidases
Metallothionein
Heavy Metals
Cadmium
Escherichia coli
Bacteria
Fusion reactions
Cell membranes
Suspensions
Genes
Cells
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bacterium-based heavy metal biosorbents : Enhanced uptake of cadmium by E. coli Expressing a metallothionein fused to β-galactosidase. / Yoshida, Naoto; Kato, T.; Yoshida, T.; Ogawa, K.; Yamashita, Mitsuo; Murooka, Y.

In: BioTechniques, Vol. 32, No. 3, 2002, p. 551-558.

Research output: Contribution to journalArticle

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