Batch growth kinetic studies of locally isolated cyanide-degrading Serratia marcescens strain AQ07

Kabiru Ibrahim Karamba, Siti Aqlima Ahmad, Azham Bin Zulkharnain, Nur Adeela Yasid, Salihu Ibrahim, Mohd Yunus Shukor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The evaluation of degradation and growth kinetics of Serratia marcescens strain AQ07 was carried out using three half-order models at all the initial concentrations of cyanide with the values of regression exceeding 0.97. The presence of varying cyanide concentrations reveals that the growth and degradation of bacteria were affected by the increase in cyanide concentration with a total halt at 700 ppm KCN after 72 h incubation. In this study, specific growth and degradation rates were found to trail the substrate inhibition kinetics. These two rates fitted well to the kinetic models of Teissier, Luong, Aiba and Heldane, while the performance of Monod model was found to be unsatisfactory. These models were used to clarify the substrate inhibition on the bacteria growth. The analyses of these models have shown that Luong model has fitted the experimental data with the highest coefficient of determination (R2) value of 0.9794 and 0.9582 with the lowest root mean square error (RMSE) value of 0.000204 and 0.001, respectively, for the specific rate of degradation and growth. It is the only model that illustrates the maximum substrate concentration (Sm) of 713.4 and empirical constant (n) of 1.516. Tessier and Aiba fitted the experimental data with a R2 value of 0.8002 and 0.7661 with low RMSE of 0.0006, respectively, for specific biodegradation rate, while having a R2 value of 0.9 and RMSE of 0.001, respectively, for specific growth rate. Haldane has the lowest R2 value of 0.67 and 0.78 for specific biodegradation and growth rate with RMSE of 0.0006 and 0.002, respectively. This indicates the level of the bacteria stability in varying concentrations of cyanide and the maximum cyanide concentration it can tolerate within a specific time period. The biokinetic constant predicted from this model demonstrates a good ability of the locally isolated bacteria in cyanide remediation in industrial effluents.

Original languageEnglish
Article number11
Journal3 Biotech
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes

Fingerprint

Serratia marcescens
cyanides
cyanide
kinetics
degradation
bacterium
bacteria
biodegradation
substrate
industrial effluents
remediation
specific growth rate
incubation
rate

Keywords

  • Biodegradation
  • Cyanide
  • Kinetic models
  • Serratia marcescens

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Batch growth kinetic studies of locally isolated cyanide-degrading Serratia marcescens strain AQ07. / Karamba, Kabiru Ibrahim; Ahmad, Siti Aqlima; Bin Zulkharnain, Azham; Yasid, Nur Adeela; Ibrahim, Salihu; Shukor, Mohd Yunus.

In: 3 Biotech, Vol. 8, No. 1, 11, 01.01.2018.

Research output: Contribution to journalArticle

Karamba, Kabiru Ibrahim ; Ahmad, Siti Aqlima ; Bin Zulkharnain, Azham ; Yasid, Nur Adeela ; Ibrahim, Salihu ; Shukor, Mohd Yunus. / Batch growth kinetic studies of locally isolated cyanide-degrading Serratia marcescens strain AQ07. In: 3 Biotech. 2018 ; Vol. 8, No. 1.
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