Bidirectional functions of arsenic as a carcinogen and an anti-cancer agent in human squamous cell carcinoma

Nguyen Dinh Thang, Ichiro Yajima, Mayuko Y. Kumasaka, Masashi Kato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bidirectional cancer-promoting and anti-cancer effects of arsenic for cancer cells have been revealed in previous studies. However, each of these effects (cancer-promoting or anti-cancer) was found in different cells at different treatedconcentration of arsenic. In this study, we for the first time indicated that arsenic at concentration of 3 μM, equal to average concentration in drinking water in cancer-prone areas in Bangladesh, simultaneously expressed its bidirectional effects on human squamous cell carcinoma HSC5 cells with distinct pathways. Treatment with 3 μM of arsenic promoted cell invasion via upregulation of expression of MT1-MMP and downregulation of expression of p14ARF and simultaneously induced cell apoptosis through inhibition of expression of N-cadherin and increase of expression of p21(WAF1/CIP1) at both transcript and protein levels in HSC5 cells. We also showed that inhibition of MT1-MMP expression by NSC405020 resulted in decrease of arsenic-mediated invasion of HSC5 cells involving decrease in phosphorylated extracellular signal-regulated kinases (pERK). Taken together, our biological and biochemical findings suggested that arsenic expressed bidirectional effects as a carcinogen and an anti-cancer agent in human squamous cell carcinoma HSC5 cells with distinct pathways. Our results might play an important scientific evident for further studies to find out a better way in treatment of arsenic-induced cancers, especially in squamous cell carcinoma.

Original languageEnglish
Article numbere96945
JournalPLoS ONE
Volume9
Issue number5
DOIs
Publication statusPublished - 2014 May 9
Externally publishedYes

Fingerprint

antineoplastic agents
squamous cell carcinoma
Arsenic
carcinogens
arsenic
Carcinogens
neoplasms
Matrix Metalloproteinase 14
cell invasion
cells
Tumor Suppressor Protein p14ARF
cadherins
Extracellular Signal-Regulated MAP Kinases
Cadherins
Epithelial Cells
Bangladesh
mitogen-activated protein kinase
Drinking Water
drinking water
apoptosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Bidirectional functions of arsenic as a carcinogen and an anti-cancer agent in human squamous cell carcinoma. / Thang, Nguyen Dinh; Yajima, Ichiro; Kumasaka, Mayuko Y.; Kato, Masashi.

In: PLoS ONE, Vol. 9, No. 5, e96945, 09.05.2014.

Research output: Contribution to journalArticle

Thang, Nguyen Dinh ; Yajima, Ichiro ; Kumasaka, Mayuko Y. ; Kato, Masashi. / Bidirectional functions of arsenic as a carcinogen and an anti-cancer agent in human squamous cell carcinoma. In: PLoS ONE. 2014 ; Vol. 9, No. 5.
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