Bio-fluorometric realtime gas-imaging system (sniff-cam) for skin ethanol vapor

Kenta Iitani, Toshiyuki Sato, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed gas-imaging system (sniff-cam) that allowed imaging of gaseous ethanol (EtOH). In the EtOH sniff-cam, a nicotinamide adenine dinucleotide (NAD)-dependent alcohol dehydrogenase (ADH) was used. When applying gaseous EtOH to an ADH-immobilized mesh with NAD+ solution, NADH was produced through an ADH-mediated reaction. NADH emits fluorescence at wavelength of 490 nm by UV excitation at 340 nm; thus concentration distribution of EtOH was visualized by measuring the distribution of fluorescence light intensity from NADH on the ADH-immobilized mesh surface. The dynamic range of EtOH sniff-cam was 0.1-1000 ppm. Besides, rounded fluorescence gradient centered in the gas outlet was observed. Results suggested that the EtOH sniff-cam could be applied to investigate the spatiotemporal change of EtOH emanated from the skin.

Original languageEnglish
Title of host publicationISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538683279
DOIs
Publication statusPublished - 2019 May
Externally publishedYes
Event18th International Symposium on Olfaction and Electronic Nose, ISOEN 2019 - Fukuoka, Japan
Duration: 2019 May 262019 May 29

Publication series

NameISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings

Conference

Conference18th International Symposium on Olfaction and Electronic Nose, ISOEN 2019
Country/TerritoryJapan
CityFukuoka
Period19/5/2619/5/29

Keywords

  • Fluorescence
  • UV-LED
  • alcohol oxidase
  • ethanol
  • gas-imaging

ASJC Scopus subject areas

  • Signal Processing
  • Instrumentation

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