Biodegradation of phenol by cold-adapted bacteria from Antarctic soils

Gillian Li Yin Lee, Siti Aqlima Ahmad, Nur Adeela Yasid, Azham Bin Zulkharnain, Peter Convey, Wan Lutfi Wan Johari, Siti Aisyah Alias, Gerardo Gonzalez-Rocha, Mohd Yunus Shukor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is a great challenge due to the harsh environmental conditions. To date, there is little information available concerning the biodegradation of phenol by indigenous Antarctic bacteria. This study addresses the isolation of three phenol-degrading bacterial strains from King George Island, Antarctica. Based on preliminary screening, three isolates (AQ5-05, AQ5-06 and AQ5-07) capable of completely degrading 0.5 g/L phenol within 120 h at 10 °C were selected for detailed study. Two were identified as Arthrobacter spp., and one Rhodococcus sp., based on 16S rRNA sequences. All strains were non-motile, Gram positive, oxidase negative and catalase positive. A study on the effects of parameters including temperature, pH, salinity and nitrogen source was conducted to optimise the conditions for phenol degradation. This revealed that the three isolates were psychrotolerant with the optimum temperature for phenol degradation between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol over a wide range of low temperatures.

Original languageEnglish
Pages (from-to)553-562
Number of pages10
JournalPolar Biology
Volume41
Issue number3
DOIs
Publication statusPublished - 2018 Mar 1
Externally publishedYes

Fingerprint

biodegradation
phenol
bacteria
soil
degradation
Rhodococcus (bacteria)
Arthrobacter
temperature
cold zones
bioremediation
Antarctica
hydrocarbons
catalase
pollutants
ribosomal RNA
salinity
screening
environmental factors
nitrogen

Keywords

  • Arthrobacter
  • Bioremediation
  • One-factor-at-a-time
  • Psychrotolerant
  • Rhodococcus
  • South Shetland Islands

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Lee, G. L. Y., Ahmad, S. A., Yasid, N. A., Bin Zulkharnain, A., Convey, P., Wan Johari, W. L., ... Shukor, M. Y. (2018). Biodegradation of phenol by cold-adapted bacteria from Antarctic soils. Polar Biology, 41(3), 553-562. https://doi.org/10.1007/s00300-017-2216-y

Biodegradation of phenol by cold-adapted bacteria from Antarctic soils. / Lee, Gillian Li Yin; Ahmad, Siti Aqlima; Yasid, Nur Adeela; Bin Zulkharnain, Azham; Convey, Peter; Wan Johari, Wan Lutfi; Alias, Siti Aisyah; Gonzalez-Rocha, Gerardo; Shukor, Mohd Yunus.

In: Polar Biology, Vol. 41, No. 3, 01.03.2018, p. 553-562.

Research output: Contribution to journalArticle

Lee, GLY, Ahmad, SA, Yasid, NA, Bin Zulkharnain, A, Convey, P, Wan Johari, WL, Alias, SA, Gonzalez-Rocha, G & Shukor, MY 2018, 'Biodegradation of phenol by cold-adapted bacteria from Antarctic soils', Polar Biology, vol. 41, no. 3, pp. 553-562. https://doi.org/10.1007/s00300-017-2216-y
Lee, Gillian Li Yin ; Ahmad, Siti Aqlima ; Yasid, Nur Adeela ; Bin Zulkharnain, Azham ; Convey, Peter ; Wan Johari, Wan Lutfi ; Alias, Siti Aisyah ; Gonzalez-Rocha, Gerardo ; Shukor, Mohd Yunus. / Biodegradation of phenol by cold-adapted bacteria from Antarctic soils. In: Polar Biology. 2018 ; Vol. 41, No. 3. pp. 553-562.
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