Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice

Rie Mukai, Itsuko Fukuda, Shin Nishiumi, Midori Natsume, Naomi Osakabe, Ken Ichi Yoshida, Hitoshi Ashida

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.

Original languageEnglish
Pages (from-to)10399-10405
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number21
DOIs
Publication statusPublished - 2008 Nov 12
Externally publishedYes

Fingerprint

Aryl Hydrocarbon Receptors
Polyphenols
hydrocarbons
polyphenols
Methylcholanthrene
receptors
mice
extracts
Dioxins
dioxins
Aryl Hydrocarbon Receptor Nuclear Translocator
Level control
Plant Extracts
Phytochemicals
Cytochromes
Nutrition
Metabolites
Glutathione Transferase
body weight
NAD(P)H dehydrogenase (quinone)

Keywords

  • Aryl hydrocarbon receptor
  • Cacao polyphenol extract
  • Cytochrome P4501A1
  • Glutathione S-transferase
  • Nad(p)h: quinone-oxidoreductase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice. / Mukai, Rie; Fukuda, Itsuko; Nishiumi, Shin; Natsume, Midori; Osakabe, Naomi; Yoshida, Ken Ichi; Ashida, Hitoshi.

In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 21, 12.11.2008, p. 10399-10405.

Research output: Contribution to journalArticle

Mukai, Rie ; Fukuda, Itsuko ; Nishiumi, Shin ; Natsume, Midori ; Osakabe, Naomi ; Yoshida, Ken Ichi ; Ashida, Hitoshi. / Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice. In: Journal of Agricultural and Food Chemistry. 2008 ; Vol. 56, No. 21. pp. 10399-10405.
@article{6bcacfbe2f334a27b05e8d181de34946,
title = "Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice",
abstract = "Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.",
keywords = "Aryl hydrocarbon receptor, Cacao polyphenol extract, Cytochrome P4501A1, Glutathione S-transferase, Nad(p)h: quinone-oxidoreductase",
author = "Rie Mukai and Itsuko Fukuda and Shin Nishiumi and Midori Natsume and Naomi Osakabe and Yoshida, {Ken Ichi} and Hitoshi Ashida",
year = "2008",
month = "11",
day = "12",
doi = "10.1021/jf802453t",
language = "English",
volume = "56",
pages = "10399--10405",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "21",

}

TY - JOUR

T1 - Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice

AU - Mukai, Rie

AU - Fukuda, Itsuko

AU - Nishiumi, Shin

AU - Natsume, Midori

AU - Osakabe, Naomi

AU - Yoshida, Ken Ichi

AU - Ashida, Hitoshi

PY - 2008/11/12

Y1 - 2008/11/12

N2 - Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.

AB - Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.

KW - Aryl hydrocarbon receptor

KW - Cacao polyphenol extract

KW - Cytochrome P4501A1

KW - Glutathione S-transferase

KW - Nad(p)h: quinone-oxidoreductase

UR - http://www.scopus.com/inward/record.url?scp=58149345878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149345878&partnerID=8YFLogxK

U2 - 10.1021/jf802453t

DO - 10.1021/jf802453t

M3 - Article

VL - 56

SP - 10399

EP - 10405

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 21

ER -