Abstract
Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.
| Original language | English |
|---|---|
| Pages (from-to) | 10399-10405 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 56 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - 2008 Nov 12 |
| Externally published | Yes |
Fingerprint
Keywords
- Aryl hydrocarbon receptor
- Cacao polyphenol extract
- Cytochrome P4501A1
- Glutathione S-transferase
- Nad(p)h: quinone-oxidoreductase
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
Cite this
Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice. / Mukai, Rie; Fukuda, Itsuko; Nishiumi, Shin; Natsume, Midori; Osakabe, Naomi; Yoshida, Ken Ichi; Ashida, Hitoshi.
In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 21, 12.11.2008, p. 10399-10405.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice
AU - Mukai, Rie
AU - Fukuda, Itsuko
AU - Nishiumi, Shin
AU - Natsume, Midori
AU - Osakabe, Naomi
AU - Yoshida, Ken Ichi
AU - Ashida, Hitoshi
PY - 2008/11/12
Y1 - 2008/11/12
N2 - Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.
AB - Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.
KW - Aryl hydrocarbon receptor
KW - Cacao polyphenol extract
KW - Cytochrome P4501A1
KW - Glutathione S-transferase
KW - Nad(p)h: quinone-oxidoreductase
UR - http://www.scopus.com/inward/record.url?scp=58149345878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149345878&partnerID=8YFLogxK
U2 - 10.1021/jf802453t
DO - 10.1021/jf802453t
M3 - Article
C2 - 18928297
AN - SCOPUS:58149345878
VL - 56
SP - 10399
EP - 10405
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 21
ER -