Calcium measurement method by fluorescent analyses using a glass micro injector

Sumito Nagasawa; I. Shimoyama

doi:10.1109/SENSOR.2003.1215265

Calcium measurement method by fluorescent analyses using a glass micro injector

Sumito Nagasawa, I. Shimoyama

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.

Original language	English
Title of host publication	TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	111-114
Number of pages	4
Volume	1
ISBN (Print)	0780377311, 9780780377318
DOIs	https://doi.org/10.1109/SENSOR.2003.1215265
Publication status	Published - 2003
Externally published	Yes
Event	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States Duration: 2003 Jun 8 → 2003 Jun 12

Other

Other	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
Country	United States
City	Boston
Period	03/6/8 → 03/6/12

Fingerprint

Keywords

Apertures
Area measurement
Calcium
Fluorescence
Glass
Informatics
Micromechanical devices
Minimally invasive surgery
Optical fiber sensors
Probes

ASJC Scopus subject areas

Electrical and Electronic Engineering

Cite this

Nagasawa, S., & Shimoyama, I. (2003). Calcium measurement method by fluorescent analyses using a glass micro injector. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers (Vol. 1, pp. 111-114). [1215265] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SENSOR.2003.1215265

Calcium measurement method by fluorescent analyses using a glass micro injector. / Nagasawa, Sumito; Shimoyama, I.

TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Vol. 1 Institute of Electrical and Electronics Engineers Inc., 2003. p. 111-114 1215265.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nagasawa, S & Shimoyama, I 2003, Calcium measurement method by fluorescent analyses using a glass micro injector. in TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. vol. 1, 1215265, Institute of Electrical and Electronics Engineers Inc., pp. 111-114, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 03/6/8. https://doi.org/10.1109/SENSOR.2003.1215265

Nagasawa S, Shimoyama I. Calcium measurement method by fluorescent analyses using a glass micro injector. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Vol. 1. Institute of Electrical and Electronics Engineers Inc. 2003. p. 111-114. 1215265 https://doi.org/10.1109/SENSOR.2003.1215265

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abstract = "We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.",

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Access to Document

10.1109/SENSOR.2003.1215265