Abstract
We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.
| Original language | English |
|---|---|
| Title of host publication | TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 111-114 |
| Number of pages | 4 |
| Volume | 1 |
| ISBN (Print) | 0780377311, 9780780377318 |
| DOIs | |
| Publication status | Published - 2003 |
| Externally published | Yes |
| Event | 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States Duration: 2003 Jun 8 → 2003 Jun 12 |
Other
| Other | 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers |
|---|---|
| Country | United States |
| City | Boston |
| Period | 03/6/8 → 03/6/12 |
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Keywords
- Apertures
- Area measurement
- Calcium
- Fluorescence
- Glass
- Informatics
- Micromechanical devices
- Minimally invasive surgery
- Optical fiber sensors
- Probes
ASJC Scopus subject areas
- Electrical and Electronic Engineering
Cite this
Calcium measurement method by fluorescent analyses using a glass micro injector. / Nagasawa, Sumito; Shimoyama, I.
TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Vol. 1 Institute of Electrical and Electronics Engineers Inc., 2003. p. 111-114 1215265.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Calcium measurement method by fluorescent analyses using a glass micro injector
AU - Nagasawa, Sumito
AU - Shimoyama, I.
PY - 2003
Y1 - 2003
N2 - We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.
AB - We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.
KW - Apertures
KW - Area measurement
KW - Calcium
KW - Fluorescence
KW - Glass
KW - Informatics
KW - Micromechanical devices
KW - Minimally invasive surgery
KW - Optical fiber sensors
KW - Probes
UR - http://www.scopus.com/inward/record.url?scp=33750120269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750120269&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2003.1215265
DO - 10.1109/SENSOR.2003.1215265
M3 - Conference contribution
AN - SCOPUS:33750120269
SN - 0780377311
SN - 9780780377318
VL - 1
SP - 111
EP - 114
BT - TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
PB - Institute of Electrical and Electronics Engineers Inc.
ER -