Calcium measurement method by fluorescent analyses using a glass micro injector

Sumito Nagasawa, I. Shimoyama

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.

Original languageEnglish
Title of host publicationTRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages111-114
Number of pages4
Volume1
ISBN (Print)0780377311, 9780780377318
DOIs
Publication statusPublished - 2003
Externally publishedYes
Event12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States
Duration: 2003 Jun 82003 Jun 12

Other

Other12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
CountryUnited States
CityBoston
Period03/6/803/6/12

Fingerprint

Calcium
Glass
Dyes
Fluorescence
Photobleaching
MEMS
Diodes
Electrodes
Fibers
Experiments

Keywords

  • Apertures
  • Area measurement
  • Calcium
  • Fluorescence
  • Glass
  • Informatics
  • Micromechanical devices
  • Minimally invasive surgery
  • Optical fiber sensors
  • Probes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Nagasawa, S., & Shimoyama, I. (2003). Calcium measurement method by fluorescent analyses using a glass micro injector. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers (Vol. 1, pp. 111-114). [1215265] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SENSOR.2003.1215265

Calcium measurement method by fluorescent analyses using a glass micro injector. / Nagasawa, Sumito; Shimoyama, I.

TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Vol. 1 Institute of Electrical and Electronics Engineers Inc., 2003. p. 111-114 1215265.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nagasawa, S & Shimoyama, I 2003, Calcium measurement method by fluorescent analyses using a glass micro injector. in TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. vol. 1, 1215265, Institute of Electrical and Electronics Engineers Inc., pp. 111-114, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 03/6/8. https://doi.org/10.1109/SENSOR.2003.1215265
Nagasawa S, Shimoyama I. Calcium measurement method by fluorescent analyses using a glass micro injector. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Vol. 1. Institute of Electrical and Electronics Engineers Inc. 2003. p. 111-114. 1215265 https://doi.org/10.1109/SENSOR.2003.1215265
Nagasawa, Sumito ; Shimoyama, I. / Calcium measurement method by fluorescent analyses using a glass micro injector. TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Vol. 1 Institute of Electrical and Electronics Engineers Inc., 2003. pp. 111-114
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