Cancer cell separator using size-dependent filtration in microfluidic chip

Taisuke Masuda, Miyako Niimi, Hayao Nakanishi, Yoko Yamanishi, Fumihito Arai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have developed a microfluidic chip that can separate gastric cancer cells from peritoneal washes. Gastric cancer cells tend to be agglomerated in the peritoneal wash, and are larger than the other cells and the soft tissue fragments. This paper presents a novel microfluidic chip to enrich low-concentrated cancer cells from the peritoneal wash by size-dependent cell filtration. This microfluidic chip has the advantage that it can separate agglomerated cells in high speed because larger objects are filtered by a gap in the microchannel. Two-step exposure was performed to fabricate a precise uneven channel for cell filtration. The main and shallow channels have heights of 100 μm and 8 μm, respectively. Furthermore, inserting fins in the microchannel that assist sheath flow enhanced continuous and robust separation against flow fluctuation in the microchannel. The microfluidic channel was designed to have high filtration efficiency and a high throughput while having a compact profile. We used patient's peritoneal washes to isolate cancer cells. As a result, we were able to enrich the cancer cells in peritoneal wash.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume185
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

separators
Separators
Microfluidics
cancer
chips
Cells
microchannels
Microchannels
cells
flow separation
fins
sheaths
Throughput
high speed
fragments
Tissue
profiles

Keywords

  • Cell sorter
  • Enrichment
  • Gastric cancer
  • Microfluidic chip
  • Peritoneal washes

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Cancer cell separator using size-dependent filtration in microfluidic chip. / Masuda, Taisuke; Niimi, Miyako; Nakanishi, Hayao; Yamanishi, Yoko; Arai, Fumihito.

In: Sensors and Actuators, B: Chemical, Vol. 185, 2013, p. 245-251.

Research output: Contribution to journalArticle

Masuda, Taisuke ; Niimi, Miyako ; Nakanishi, Hayao ; Yamanishi, Yoko ; Arai, Fumihito. / Cancer cell separator using size-dependent filtration in microfluidic chip. In: Sensors and Actuators, B: Chemical. 2013 ; Vol. 185. pp. 245-251.
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