Carbon nanotube-plasma polymer-based amperometric biosensors: Enzyme-friendly platform for ultrasensitive glucose detection

Hitoshi Muguruma, Yasunori Matsui, Yu Shibayama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An amperometric enzyme biosensor fabricated with carbon nanotubes (CNTs) and plasma-polymerized thin films (PPFs) is reported. A mixture of the enzyme glucose oxidase (GOD) and a CNT film is sandwiched with 10-nm-thick acetonitrile PPFs. Under PPF layer was deposited onto a sputtered gold electrode. To facilitate the electrochemical communication between the CNT layer and GOD, CNT was treated with oxygen plasma. The device with single-walled CNTs showed a sensitivity higher than that of multi walled CNTs. The glucose biosensor showed ultrasensitivity (a sensitivity of 40μA mM-1 cm-2, a correlation coefficient of 0.992, a linear response range of 0.025 -1.9 mM, a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (<4 s in reaching 95% of maximum response). This high performance is attributed to the fact that CNTs have excellent electrocatalytic activity and enhance electron transfer, and that PPFs and/or the plasma process for CNTs are the enzyme-friendly platform, i.e., a suitable design of the interface between GOD and CNTs.

Original languageEnglish
Pages (from-to)6078-6082
Number of pages5
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume46
Issue number9 A
DOIs
Publication statusPublished - 2007 Sep 7

Fingerprint

bioinstrumentation
Biosensors
glucose
Glucose
enzymes
Carbon nanotubes
Enzymes
platforms
carbon nanotubes
Plasmas
polymers
Polymers
Glucose oxidase
oxidase
Thin films
thin films
Single-walled carbon nanotubes (SWCN)
Acetonitrile
sensitivity
oxygen plasma

Keywords

  • Carbon nanotube
  • Electrochemistry
  • Enzyme
  • Glucose
  • Plasma polymer

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

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abstract = "An amperometric enzyme biosensor fabricated with carbon nanotubes (CNTs) and plasma-polymerized thin films (PPFs) is reported. A mixture of the enzyme glucose oxidase (GOD) and a CNT film is sandwiched with 10-nm-thick acetonitrile PPFs. Under PPF layer was deposited onto a sputtered gold electrode. To facilitate the electrochemical communication between the CNT layer and GOD, CNT was treated with oxygen plasma. The device with single-walled CNTs showed a sensitivity higher than that of multi walled CNTs. The glucose biosensor showed ultrasensitivity (a sensitivity of 40μA mM-1 cm-2, a correlation coefficient of 0.992, a linear response range of 0.025 -1.9 mM, a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (<4 s in reaching 95{\%} of maximum response). This high performance is attributed to the fact that CNTs have excellent electrocatalytic activity and enhance electron transfer, and that PPFs and/or the plasma process for CNTs are the enzyme-friendly platform, i.e., a suitable design of the interface between GOD and CNTs.",
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AB - An amperometric enzyme biosensor fabricated with carbon nanotubes (CNTs) and plasma-polymerized thin films (PPFs) is reported. A mixture of the enzyme glucose oxidase (GOD) and a CNT film is sandwiched with 10-nm-thick acetonitrile PPFs. Under PPF layer was deposited onto a sputtered gold electrode. To facilitate the electrochemical communication between the CNT layer and GOD, CNT was treated with oxygen plasma. The device with single-walled CNTs showed a sensitivity higher than that of multi walled CNTs. The glucose biosensor showed ultrasensitivity (a sensitivity of 40μA mM-1 cm-2, a correlation coefficient of 0.992, a linear response range of 0.025 -1.9 mM, a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (<4 s in reaching 95% of maximum response). This high performance is attributed to the fact that CNTs have excellent electrocatalytic activity and enhance electron transfer, and that PPFs and/or the plasma process for CNTs are the enzyme-friendly platform, i.e., a suitable design of the interface between GOD and CNTs.

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