Cathodic electrochemiluminescence of luminol enhanced by antibody-antigen reaction

Yasuo Yoshimi, Hirotaka Haramoto, Takehiro Miyasaka, Kiyotaka Sakai

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13 Citations (Scopus)


The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemiluminescent intensity of luminol resulted from antigen-antibody reactions. The cathodic electrochemiluminescences of luminol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic electrochemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous immunoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presence of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while luminol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.

Original languageEnglish
Pages (from-to)851-857
Number of pages7
JournalJournal of Chemical Engineering of Japan
Issue number5
Publication statusPublished - 1996 Oct
Externally publishedYes


  • Antibody
  • Antigen
  • Biochemical Engineering
  • Electrochemiluminescence
  • Luminol

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


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