Abstract
The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemiluminescent intensity of luminol resulted from antigen-antibody reactions. The cathodic electrochemiluminescences of luminol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic electrochemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous immunoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presence of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while luminol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.
| Original language | English |
|---|---|
| Pages (from-to) | 851-857 |
| Number of pages | 7 |
| Journal | Journal of Chemical Engineering of Japan |
| Volume | 29 |
| Issue number | 5 |
| Publication status | Published - 1996 Oct |
| Externally published | Yes |
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Keywords
- Antibody
- Antigen
- Biochemical Engineering
- Electrochemiluminescence
- Luminol
ASJC Scopus subject areas
- Chemical Engineering(all)
- Chemistry(all)
Cite this
Cathodic electrochemiluminescence of luminol enhanced by antibody-antigen reaction. / Yoshimi, Yasuo; Haramoto, Hirotaka; Miyasaka, Takehiro; Sakai, Kiyotaka.
In: Journal of Chemical Engineering of Japan, Vol. 29, No. 5, 10.1996, p. 851-857.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cathodic electrochemiluminescence of luminol enhanced by antibody-antigen reaction
AU - Yoshimi, Yasuo
AU - Haramoto, Hirotaka
AU - Miyasaka, Takehiro
AU - Sakai, Kiyotaka
PY - 1996/10
Y1 - 1996/10
N2 - The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemiluminescent intensity of luminol resulted from antigen-antibody reactions. The cathodic electrochemiluminescences of luminol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic electrochemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous immunoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presence of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while luminol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.
AB - The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemiluminescent intensity of luminol resulted from antigen-antibody reactions. The cathodic electrochemiluminescences of luminol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic electrochemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous immunoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presence of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while luminol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.
KW - Antibody
KW - Antigen
KW - Biochemical Engineering
KW - Electrochemiluminescence
KW - Luminol
UR - http://www.scopus.com/inward/record.url?scp=0000241935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000241935&partnerID=8YFLogxK
M3 - Article
VL - 29
SP - 851
EP - 857
JO - Journal of Chemical Engineering of Japan
JF - Journal of Chemical Engineering of Japan
SN - 0021-9592
IS - 5
ER -