Characterization of Pseudomonas stutzeri NT-I capable of removing soluble selenium from the aqueous phase under aerobic conditions

Masashi Kuroda, Emi Notaguchi, Akiko Sato, Masaya Yoshioka, Ai Hasegawa, Tsubasa Kagami, Takanobu Narita, Mitsuo Yamashita, Kazunari Sei, Satoshi Soda, Michihiko Ike

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Pseudomonas stutzeri strain NT-I was isolated from the drainage wastewater of a selenium refinery plant. This bacterium efficiently reduced selenate to elemental selenium without prolonged accumulation of selenite under aerobic conditions. Strain NT-I was able to reduce selenate completely at high concentrations (up to 10mM) and selenite almost completely (up to 9mM). In addition, higher concentrations of selenate and selenite were substantially reduced. Activity was observed under the following experimental conditions: 20-50°C, pH 7-9, and 0.05-20g L -1 NaCl for selenate reduction, and 20-50°C, pH 6-9, and 0.05-50g L -1 NaCl for selenite reduction. Under anaerobic conditions, selenate was reduced more rapidly, whereas selenite was not reduced at all. The high selenate- and selenite-reducing capability at high concentrations suggested that strain NT-I is suitable for the removal of selenium from high-strength industrial wastewater.

Original languageEnglish
Pages (from-to)259-264
Number of pages6
JournalJournal of Bioscience and Bioengineering
Issue number3
Publication statusPublished - 2011 Sep 1



  • Elemental selenium
  • Industrial wastewater
  • Pseudomonas stutzeri
  • Selenate
  • Selenite
  • Selenium removal
  • Specific growth rate
  • Specific reduction rate

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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