Abstract
We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.
| Original language | English |
|---|---|
| Pages (from-to) | 139-144 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 145 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1994 Jul 22 |
| Externally published | Yes |
Keywords
- catalytic core
- in vitro splicing
- LSU rRNA
- reverse splicing
- Ribozyme
- salt activation
- salt inhibition
- SSU rRNA
ASJC Scopus subject areas
- Genetics
Cite this
Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87. / Tadanori, Aimi; Takashi, Yamada; Yamashita, Mitsuo; Yoshikatsu, Murooka.
In: Gene, Vol. 145, No. 1, 22.07.1994, p. 139-144.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87
AU - Tadanori, Aimi
AU - Takashi, Yamada
AU - Yamashita, Mitsuo
AU - Yoshikatsu, Murooka
PY - 1994/7/22
Y1 - 1994/7/22
N2 - We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.
AB - We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.
KW - catalytic core
KW - in vitro splicing
KW - LSU rRNA
KW - reverse splicing
KW - Ribozyme
KW - salt activation
KW - salt inhibition
KW - SSU rRNA
UR - http://www.scopus.com/inward/record.url?scp=0028306586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028306586&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(94)90337-9
DO - 10.1016/0378-1119(94)90337-9
M3 - Article
C2 - 8045414
AN - SCOPUS:0028306586
VL - 145
SP - 139
EP - 144
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -