Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87

Aimi Tadanori; Yamada Takashi; Yamashita Mitsuo; Murooka Yoshikatsu

doi:10.1016/0378-1119(94)90337-9

Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87

Aimi Tadanori, Yamada Takashi, Yamashita Mitsuo, Murooka Yoshikatsu

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Gene
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90337-9
Publication status	Published - 1994 Jul 22
Externally published	Yes

Keywords

LSU rRNA
Ribozyme
SSU rRNA
catalytic core
in vitro splicing
reverse splicing
salt activation
salt inhibition

ASJC Scopus subject areas

Genetics

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title = "Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87",

abstract = "We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.",

keywords = "LSU rRNA, Ribozyme, SSU rRNA, catalytic core, in vitro splicing, reverse splicing, salt activation, salt inhibition",

author = "Aimi Tadanori and Yamada Takashi and Yamashita Mitsuo and Murooka Yoshikatsu",

year = "1994",

month = jul,

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doi = "10.1016/0378-1119(94)90337-9",

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journal = "Gene",

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TY - JOUR

T1 - Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87

AU - Tadanori, Aimi

AU - Takashi, Yamada

AU - Mitsuo, Yamashita

AU - Yoshikatsu, Murooka

PY - 1994/7/22

Y1 - 1994/7/22

N2 - We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.

AB - We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.

KW - LSU rRNA

KW - Ribozyme

KW - SSU rRNA

KW - catalytic core

KW - in vitro splicing

KW - reverse splicing

KW - salt activation

KW - salt inhibition

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