Chlamydomonas CAV2 Encodes a Voltage- Dependent Calcium Channel Required for the Flagellar Waveform Conversion

Kenta Fujiu, Yoshitaka Nakayama, Ayaka Yanagisawa, Masahiro Sokabe, Kenjiro Yoshimura

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Cilia and flagella can alter their beating patterns through changes in membrane excitation mediated by Ca2+ influx [1-6]. The ion channel that generates this Ca2+ influx and its cellular distribution have not been identified. In this study, we analyzed the Chlamydomonas ppr2 mutant, which is deficient in the production of a flagellar Ca2+ current and consequently has a defective photophobic response [7] and mechanoshock response. ppr2 had a mutation in CAV2, which encodes a homolog of the α1 subunit of voltage-dependent calcium channels (VDCCs). CAV2 has four domains, each with six transmembrane segments and EEEE loci in the ion-selective filter, which are typical of VDCCs in vertebrates. Interestingly, we found that CAV2 primarily localized toward the distal part of flagella. We provide evidence that CAV2 is transported toward the flagellar tip via intraflagellar transport (IFT) because CAV2 accumulated near the flagellar base when IFT was blocked. The results of this study suggest that the Ca2+ influx of Chlamydomonas flagella is mediated by the VDCC, CAV2, whose distribution is biased to the distal region of the flagellum.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalCurrent Biology
Volume19
Issue number2
DOIs
Publication statusPublished - 2009 Jan 27
Externally publishedYes

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calcium channels
Chlamydomonas
Calcium Channels
flagellum
calcium
Ion Channels
ion channels
cilia
Ions
Membranes
vertebrates
ions
mutation
mutants
loci

Keywords

  • CELLBIO
  • PHYSIOLOGY
  • SIGNALING

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chlamydomonas CAV2 Encodes a Voltage- Dependent Calcium Channel Required for the Flagellar Waveform Conversion. / Fujiu, Kenta; Nakayama, Yoshitaka; Yanagisawa, Ayaka; Sokabe, Masahiro; Yoshimura, Kenjiro.

In: Current Biology, Vol. 19, No. 2, 27.01.2009, p. 133-139.

Research output: Contribution to journalArticle

Fujiu, Kenta ; Nakayama, Yoshitaka ; Yanagisawa, Ayaka ; Sokabe, Masahiro ; Yoshimura, Kenjiro. / Chlamydomonas CAV2 Encodes a Voltage- Dependent Calcium Channel Required for the Flagellar Waveform Conversion. In: Current Biology. 2009 ; Vol. 19, No. 2. pp. 133-139.
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